Glucuronic Acid Epimerase 293T Cell Transient Overexpression Lysate

GLCE transfected lysate.

Lane 1: GLCE transfected lysate (67.98 KDa) Lane 2: Non-transfected lysate.

Product Discontinued

Glucuronic Acid Epimerase 293T Cell Transient Overexpression Lysate Summary

• Description: Quality control test: Transient overexpression cell lysate was tested with Anti-GLCE antibody by Western Blots. Plasmid: pCMV-GLCE full-length
• Specificity: Glucuronic Acid Epimerase 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: GLCE

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Glucuronic Acid Epimerase 293T Cell Transient Overexpression Lysate

• D-glucuronyl C5-epimerase
• EC 5.1.3
• EC 5.1.3.-
• glucuronic acid epimerase
• glucuronyl C5-epimerase
• Heparin/heparan sulfate:glucuronic acid C5-epimerase
• HSEPIheparin/heparan sulfate-glucuronic acid C5-epimerase
• KIAA0836heparan sulfate epimerase
• UDP-glucuronic acid epimerase

Background

Heparan sulfate (HS) is a negatively charged cell surface polysaccharide required for the biologic activities of circulating extracellular ligands. GLCE is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of HS, which endows the nascent polysaccharide chain with the ability to bind growth factors and cytokines (Ghiselli and Agrawal, 2005 [PubMed 15853773]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: GLCE