Glucosylceramidase/GBA Colorimetric Assay Kit

Images

 
Enzyme Activity: Glucosylceramidase/GBA Colorimetric Assay Kit [KA1611] - Kinetics of beta-glucosidase reaction in 96-well plate assay.

Product Details

Summary
Kit Type
Colorimetric Assay Kit

Order Details

Glucosylceramidase/GBA Colorimetric Assay Kit Summary

Description
beta-Glucosidase Assay Kit is a colorimetric kinetic determination of beta-Glucosidase activity.
Kit Type
Colorimetric Assay Kit
Gene
GBA

Applications/Dilutions

Publications
Read Publication using KA1611.

Packaging, Storage & Formulations

Storage
Store at -20C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Glucosylceramidase/GBA Colorimetric Assay Kit

  • Acid beta-glucosidase
  • Alglucerase
  • beta-glucocerebrosidase
  • D-glucosyl-N-acylsphingosine glucohydrolase
  • EC 3.2.1.45
  • GBA
  • GBA1
  • GC
  • GCB
  • GLUC
  • glucosidase, beta, acid
  • glucosidase, beta; acid (includes glucosylceramidase)
  • Glucosylceramidase
  • Imiglucerase
  • lysosomal glucocerebrosidase

Background

beta-GLUCOSIDASE is a glucosidase enzyme which acts upon beta 1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). beta-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a beta-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a beta-glucosidase isoform (lysosomal gluco-cerebrosidase) has been related to Gaucher's disease and Parkinson's disease. Simple, direct and automation-ready procedures for measuring beta-glucosidase activity are becoming popular in Research and Drug Discovery. Beta-Glucosidase Assay Kit is designed to measure beta-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl- beta-D-glucopyranoside that is hydrolyzed specifically by beta-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Publications for Glucosylceramidase/GBA Kit (KA1611)(1)

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FAQs for Glucosylceramidase/GBA Kit (KA1611). (Showing 1 - 1 of 1 FAQ).

  1. Could you advise on the best way to prepare a cell lysate for use with your beta-glucosidase assay kit (KA1611)?
    • This particular product is made by a Taiwanese company called Abnova; we distribute their products in addition to selling our own. Here is a link to their protocol for KA1611. We suggest to prepare lysate in 50 mM phosphate (pH 7.0) buffer or in any other suitable enzyme buffer. However, the following chemicals are known to affect the enzyme activity and should be avoided: SH-containing reagents (e.g. dithiothreitol, 2-mercaptoethanol, glutathione), Ca2+, Cu2+, Fe3+/Fe2+, Hg2+, Mg2+, Ni2+, Zn2+, SDS, EDTA and Tris.

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Bioinformatics

Gene Symbol GBA
Entrez