Glucose 6 Phosphate Dehydrogenase Antibody (JA31-40)

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody (JA31-40) [NBP2-66870] - Analysis of Glucose 6 Phosphate Dehydrogenase on A549 cell lysate using anti-Glucose 6 Phosphate Dehydrogenase antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Glucose 6 Phosphate Dehydrogenase Antibody (JA31-40) [NBP2-66870] - Staining Glucose 6 Phosphate Dehydrogenase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (JA31-40) [NBP2-66870] - Analysis of paraffin-embedded mouse testes tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.

Flow Cytometry: Glucose 6 Phosphate Dehydrogenase Antibody (JA31-40) [NBP2-66870] - Analysis of Hela cells with Glucose 6 Phosphate Dehydrogenase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (JA31-40) [NBP2-66870] - Analysis of paraffin-embedded human liver tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (JA31-40) [NBP2-66870] - Analysis of paraffin-embedded human stomach cancer tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Glucose 6 Phosphate Dehydrogenase Antibody (JA31-40) [NBP2-66870] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Glucose 6 Phosphate Dehydrogenase antibody. Counter stained with hematoxylin.

Product Discontinued

Glucose 6 Phosphate Dehydrogenase Antibody (JA31-40) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 460-509 / 515. (SwissProt: P11413 Human)
• Localization: Cytoplasm. Cytoplasmic side of plasma membrane. Membrane. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: G6PD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Glucose 6 Phosphate Dehydrogenase Antibody (JA31-40)

• G6PD
• G6PD1EC 1.1.1.49
• G6PDH
• glucose-6-phosphate dehydrogenase

Background

Glucose-6-phosphate dehydrogenase (G6PD) is a cytosolic enzyme that catalyzes the conversion of glucose-6-phosphate into 6-phosphgluconolactone and maintains the levels of the electron donor, NADPH. G6PD is an x-linked housekeeping gene whose deficiency can result in neonatal jaundice, acute hemolysis, or chronic non-spherocytic hemolytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: G6PD