Glucose 6 Phosphate Dehydrogenase Antibody (1067503) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 1067503
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

Glucose 6 Phosphate Dehydrogenase Antibody (1067503) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived human G6PD
  Ala2-Leu515
  Accession # P11413
• Specificity: Detects human G6PD in direct ELISA.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glucose 6 Phosphate Dehydrogenase Antibody (1067503) [Alexa Fluor® 594]

• G6PD
• G6PD1EC 1.1.1.49
• G6PDH
• glucose-6-phosphate dehydrogenase

Background

Glucose-6-phosphate dehydrogenase (G6PD) converts D-glucose 6-phosphate (G6P) into 6-phosphoglucono-δ-lactone and generate co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH) (1). G6PD is the rate-limiting enzyme of the pentose phosphate pathway that supplies reducing energy to cells by maintaining the level of NADPH, which in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage from compounds like hydrogen peroxide (1, 2). More importantly, NADPH is used for biosynthesis of fatty acids or isoprenoids. G6PD is generally found as a dimer of two identical monomers (3). Depending on conditions, such as pH, these dimers can themselves dimerize to form tetramers. Each monomer in the complex has a substrate binding site that binds to G6P, and a catalytic coenzyme binding site that binds to NADP⁺/NADPH using the Rossman fold (4). Its activity is stimulated by the substrate G6P and NADP⁺. Clinically, genetic deficiency of G6PD predisposes a person to non-immune hemolytic anemia (5). G6PD is remarkable for its genetic diversity. Many variants of G6PD have been described with wide-ranging levels of enzyme activity and associated clinical symptoms. G6PD is frequently used as a coupling enzyme for measuring the enzymatic activity of glucose kinase (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.