Glucosamine (N-acetyl)-6-Sulfatase/GNS Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

Glucosamine (N-acetyl)-6-Sulfatase/GNS Antibody [Alexa Fluor® 700] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Glucosamine (N-acetyl)‑6-Sulfatase/GNS
  Val37-Leu552
  Accession # P15586
• Specificity: Detects human Glucosamine (N-acetyl)-6‑Sulfatase/GNS in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glucosamine (N-acetyl)-6-Sulfatase/GNS Antibody [Alexa Fluor® 700]

• EC 3.1.6
• EC 3.1.6.14
• G6Sglucosamine-6-sulfatase
• glucosamine (N-acetyl)-6-sulfatase
• Glucosamine6Sulfatase
• Glucosamine-6-Sulfatase
• GNS
• MGC21274
• N-acetylglucosamine-6-sulfatase

Background

A member of the sulfatase family, GNS is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and keratan sulfate (1, 2). It hydrolyzes the 6-sulfate group of the N-acetyl-D-glucosamine 6-sulfate units of the GAG. GNS deficiency results in mucopolysaccharidosis type IIID (MPS IIID or Sanfilippo D Syndrome), an inborn error leading to lysosomal accumulation of heparan sulfate. MPS IIID has profound mental deterioration, hyperactivity, and relatively mild somatic manifestations. The deduced amino acid sequence of human GNS consists of a signal peptide (residues 1‑36) and a mature chain (residues 37‑552) that may be further processed into N-terminal and C-terminal fragments (3). Recombinant human GNS corresponds to the single chain and has sulfatase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.