Glucagon R/GCGR Antibody

Western Blot: Glucagon R/GCGR Antibody [NBP1-30971] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Glucagon Receptor antibody at a dilution of 1:1000.

Immunohistochemistry-Paraffin: Glucagon R/GCGR Antibody [NBP1-30971] - Paraffin-embedded CL1-0 xenograft, using antibody at 1:100 dilution.

Western Blot: Glucagon R/GCGR Antibody [NBP1-30971] - Sample (30 ug of whole cell lysate)A: 293T 10% SDS PAGE, antibody diluted at 1:1000.

Product Discontinued

Glucagon R/GCGR Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Glucagon Receptor. The exact sequence is proprietary.
• Localization: Cell membrane, Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GCGR
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%), Bovine (87%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Glucagon R/GCGR Antibody

• FLJ97182
• GCGR
• GGR
• GLR
• GL-R
• Glucagon R
• glucagon receptor
• GlucagonR
• MGC138246

Background

Glucagon, a pancreatic hormone, functions as an antagonist to insulin, stimulating the conversion of glycogen to glucose and increasing blood sugar levels. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior. Both glucagon and GLP-1 function through their specific binding to the glucagon receptor or GLP-1R, respectively. The glucagon receptor shows expression in liver, kidney and adipose tissue. The 56 GLP-1R expression primarily localizes to areas of the hypothalamus involved in feeding behavior. Both receptors and their ligands serve as potential targets for the therapeutic treatment of diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GCGR
• Entrez:
  • 2642(Human)
• Uniprot:
  • P47871()