Glucagon R/GCGR Antibody (591929) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with (A) human Glucagon R/GCGR or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Glucagon R/GCGR Monoclonal Antibody (Catalog # MAB10296) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 591929
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Glucagon R/GCGR Antibody (591929) [Unconjugated] Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with human Glucagon R/GCGR
  Met1-Gln142
  Accession # P47871
• Specificity: Detects human Glucagon R/GCGR in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glucagon R/GCGR Antibody (591929) [Unconjugated]

• FLJ97182
• GCGR
• GGR
• GLR
• GL-R
• Glucagon R
• glucagon receptor
• GlucagonR
• MGC138246

Background

GLR (GCGR/Glucagon Receptor) is a GPCR for glucagon that plays a role in blood glucose levels and glucose homeostasis. Glucagon receptors are mainly expressed in liver and kidney but also expressed in other tissues such as heart, adipose, spleen, thymus, adrenal glands, pancreas, cerebral cortex and the gastrointestinal tract. Mutations in the GCGR gene is associated with Type 2 Diabetes Mellitus and inactivation of the receptor causes glucagon resistance. GCGR is found on the cell membrane but is rapidly internalized following ligand-binding via endocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P47871()