GLS2 Antibody (CL7721) - Azide and BSA Free

Analysis in human liver and skeletal muscle tissues using NBP3-44210 antibody. Corresponding GLS2 RNA-seq data are presented for the same tissues.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.

Staining of human prostate shows no positivity in glandular cells as expected.

Analysis in human cell line Daudi.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL7721
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GLS2 Antibody (CL7721) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a synthetic peptide of Q9UI32, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GLS2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for GLS2 Antibody (CL7721) - Azide and BSA Free

• EC 3.5.1.2
• GAbreast cell glutaminase
• GLSglutaminase GA
• glutaminase 2 (liver, mitochondrial)
• glutaminase I
• glutaminase liver isoform, mitochondrial
• hLGA
• LGA
• L-glutaminase
• L-glutamine amidohydrolase
• MGC71567
• phosphate-activated glutaminase
• phosphate-dependent glutaminase
• truncated glutaminase 2

Background

The protein encoded by the GLS2 gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. This protein is functionally similar to the kidney glutaminase but is a little smaller in size. Originally thought to be liver-specific, this protein has been found in other tissues as well. At least one transcribed pseudogene has been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GLS2