Carbonic Anhydrase VA/CA5A Antibody

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase VA/CA5A Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Carbonic Anhydrase VA/CA5A was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Carbonic Anhydrase VA/CA5A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3049) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Carbonic Anhydrase VA/CA5A Antibody Summary

Immunogen
E. coli-derived recombinant human Carbonic Anhydrase VA/CA5A
Ala40-Ser305
Accession # P35218
Specificity
Detects human Carbonic Anhydrase VA/CA5A in direct ELISAs and Western blots. In direct ELISAs, approximately 4% cross-reactivity with recombinant human (rh) CA5B is observed and less than 1% cross-reactivity with rhCA2 and rhCA7.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CA5A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase VA/CA5A Antibody

  • CA5A
  • CA5carbonic anhydrase 5A, mitochondrial
  • Carbonate dehydratase VA
  • carbonic anhydrase V, mitochondrial
  • Carbonic Anhydrase VA
  • carbonic anhydrase VA, mitochondrial
  • carbonic dehydratase
  • CAV
  • CAVA
  • CA-VA
  • EC 4.2.1.1

Background

Carbonic Anhydrase catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. Carbonic anhydrase VA encoded by the CA5A gene is a mitochondrial protein (2, 3). In comparison with another mitochondrial CA (CA5B), CA5A has different tissue distribution and chromosomal location (4, 5). Expression and inhibitor studies of different CAs in the rat pancreatic beta cells indicate that CA5A may be involved in the regulation of insulin secretion (6). CA5A may also participate in the detoxification of ammonia produced in the gastrointestinal tract by providing bicarbonate to carbamyl phosphate synthetase I (7). The amino acid sequence of recombinant human CA5A (residues 40 to 305) is 79%, 77%, and 76% identical to that of canine, bovine, and rat/mouse.

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Nagao, Y. et al. (1993) Proc. Natl. Acad. Sci. USA 90:7623.
  3. Nagao, Y. et al. (1995) Genomics 28:477.
  4. Shah, G.N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1677.
  5. Fujikawa-Adachi, K. et al. (1999) J. Biol. Chem. 274:21228.
  6. Parkkila, A.K. et al. (1998) J. Biol. Chem. 273:24620.
  7. Saarnio, J. et al. (1999) J. Histochem. Cytochem. 47:517.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-615
Species: Hu, Mu, Rt, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB110-5029
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB300-500
Species: Hu, Mu, Rt, Po, Am, Bv, Ca, Dr
Applications: WB, IHC, IHC-Fr, IHC-P
NBP1-31116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-76919
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-89228
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-33950
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
44110002
Species: Hu
Applications: WB, ELISA, ICC/IF
NB100-858
Species: Hu, Mu, Rt, GP, Mk
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-WhMt
NB100-1269
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
NB300-605
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
H00005555-P01
Species: Hu
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow

Publications for Carbonic Anhydrase VA/CA5A Antibody (AF3049) (0)

There are no publications for Carbonic Anhydrase VA/CA5A Antibody (AF3049).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Carbonic Anhydrase VA/CA5A Antibody (AF3049) (0)

There are no reviews for Carbonic Anhydrase VA/CA5A Antibody (AF3049). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Carbonic Anhydrase VA/CA5A Antibody (AF3049) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Carbonic Anhydrase VA/CA5A Products

Bioinformatics Tool for Carbonic Anhydrase VA/CA5A Antibody (AF3049)

Discover related pathways, diseases and genes to Carbonic Anhydrase VA/CA5A Antibody (AF3049). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on Carbonic Anhydrase VA/CA5A

There are no specific blogs for Carbonic Anhydrase VA/CA5A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Carbonic Anhydrase VA/CA5A Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CA5A
Entrez
Uniprot