GLI-3 Antibody

Western Blot: GLI-3 Antibody [NBP2-29627] - Adult mouse brain tissue lysates. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: GLI-3 Antibody [NBP2-29627] - HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-29627] - Analysis of in mouse liver.

Flow (Intracellular): GLI-3 Antibody [NBP2-29627] - Intracellular staining for GLI-3 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-3 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a negative control. Image courtesy of Sherine Elsawa, Northern Illinois University.

Western Blot: GLI-3 Antibody [NBP2-29627] - Analysis of GLI-3 in Hela cell lysate and MEF cell lysate.

Immunohistochemistry-Paraffin: GLI-3 Antibody [NBP2-29627] - Analysis of in mouse testis.

• Reactivity: Hu, Mu
• Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

GLI-3 Antibody Summary

• Immunogen: A synthetic peptide made to an internal portion of human Gli3 protein (between residues 50-120) [UniProt P10071]
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GLI3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Flow (Intracellular)
• Application Notes: This Gli3 antibody is useful for Western blot where bands were observed at ~75 kDa (GLI3R) in both HeLa and MEF cell lysates while an additional band was observed ~150 kDa (GLI3A) in MEF cell lysate only. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in FLOW reported in scientific literature (PMID: 26238488).
• Theoretical MW: 190 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen has 84% homology to Xenopus and 85% homology to chicken.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GLI-3 Antibody

• ACLS
• ACLSPAPAPHS
• ADD
• Bph
• GCPS
• GLI family zinc finger 3
• GLI3 form of 190 kDa
• GLI3 full length protein
• GLI3
• GLI-3
• GLI3-190
• GLI3FL
• GLI-Kruppel family member GLI3
• glioma-associated oncogene family zinc finger 3
• Greig cephalopolysyndactyly syndrome
• oncogene GLI3
• PAP-A
• PAPA1
• PAPB
• PAPBDNA-binding protein
• Pdn
• PHS
• PPDIV
• Xt
• zinc finger protein GLI3

Background

Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-polysyndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and craniofacial development. Two isoforms of human Gli-3 have been reported. One is the full length protein at ~170-190kDa and the other is a truncated isoform at ~80kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GLI-3 Antibody (NBP2-29627)(1)

Publication using NBP2-29627

• Jackson DA, Smith TD, Amarsaikhan N et al. Modulation of the IL-6 Receptor alpha Underlies GLI2-Mediated Regulation of Ig Secretion in Waldenstrom Macroglobulinemia Cells. J Immunol 2015 Sep 15 [PMID: 26238488]

Review for GLI-3 Antibody (NBP2-29627) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Zhihui Zhu WB Mouse 07/09/2019

Summary

• Application: Western Blot
• Sample Tested: mouse brain lysates,Adult mouse brain tissue
• Species: Mouse
• Lot: A-1

Comments

• Comments: mice brain samples for western blot.

Bioinformatics

• Gene Symbol: GLI3
• Entrez:
  • 2737(Human)
• Uniprot:
  • P10071()