GLI-1 Antibody [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human GLI-1. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GLI-1 at approximately 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GLI-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Numb is required for maximal activation of Hh signaling. A Diagram of the Hh signaling cascade. Numb regulates Hh signaling at the level of Ptch1; SmoM2 actives Hh signaling independent of Shh. b, c Hh signaling levels in WT or Numb KO cells were assessed by the transcript levels of the Hh-target genes, Gli1 and Ptch1, via qPCR. d Hh signaling levels in WT cells and Numb KO cells that express Numb-V5 or the blank vector. Hh activity was assessed by Gli1 transcript levels. e, f Western blot analysis and quantification of Gli1 protein levels in WT and Numb KO cells. Prior to harvesting, cells were stimulated with 1 μg/ml Shh in the low serum culture medium for 24 h. g SAG-induced Hh signaling levels in WT cells and Numb KO cells. Prior to harvesting, cells were treated with the indicated doses of SAG in the low serum culture medium for 24 h. h Hh signaling activity in WT cells and Numb KO cells infected with lentiviruses that express SmoM2. All experiments were repeated three to four times with similar results. Data are shown as mean ± SD. Statistics in (b, c, d, f, g, h): Two-way ANOVA with multiple comparisons (Tukey test). ns not significant. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38664376), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GLI-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse GLI-1
  Met1-Glu237
  Accession # P47806
• Specificity: Detects human and mouse GLI-1.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GLI1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GLI-1 Antibody [Unconjugated]

• GLI family zinc finger 1
• GLI1
• GLI-1
• glioma-associated oncogene family zinc finger 1
• glioma-associated oncogene homolog 1 (zinc finger protein)
• Glioma-associated oncogene
• GLIzinc finger protein GLI1
• Oncogene GLI

Background

GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI-1 activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GLI-1 Antibody (AF3455)(22)

Publications using AF3455

• Jangde, N;Lee, MH;Ruiz, L;Egan, I;Jermakowicz, AM;Wynn, D;Goka, E;Lippman, M;Robbins, DJ;Ayad, NG; RAC1 Regulates Shh-Medulloblastoma Growth via GLI-Mediated Transcription bioRxiv : the preprint server for biology 2025-05-23 [PMID: 40475618] (Immunocytochemistry, Mouse)
• EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6. 2022-10-21 [PMID: 36272401] (IHC, Mouse)
• Xueping Lei, Zhan Li, Yihang Zhong, Songpei Li, Jiacong Chen, Yuanyu Ke et al. Gli1 promotes epithelial–mesenchymal transition and metastasis of non-small cell lung carcinoma by regulating snail transcriptional activity and stability Acta Pharmaceutica Sinica B 2022-10-01 [PMID: 36213531] (Immunohistochemistry, Human)
• Xueping Lei, Zhan Li, Yihang Zhong, Songpei Li, Jiacong Chen, Yuanyu Ke et al. Gli1 promotes epithelial–mesenchymal transition and metastasis of non-small cell lung carcinoma by regulating snail transcriptional activity and stability Acta Pharmaceutica Sinica B 10/1/2022 [PMID: 36213531]
• M Sobecki, J Chen, E Krzywinska, S Nagarajan, Z Fan, E Nelius, JM Monné Rodr, F Seehusen, A Hussein, G Moschini, EY Hajam, R Kiran, D Gotthardt, J Debbache, C Badoual, T Sato, T Isagawa, N Takeda, C Tanchot, E Tartour, A Weber, S Werner, J Loffing, L Sommer, V Sexl, C Münz, C Feghali-Bo, E Pachera, O Distler, J Snedeker, C Jamora, C Stockmann Vaccination-based immunotherapy to target profibrotic cells in liver and lung Cell Stem Cell, 2022-09-15;0(0):. 2022-09-15 [PMID: 36113462] (Western Blot, Mouse)
• M Min, T Song, M Sun, T Wang, J Tan, J Zhang Dhh signaling pathway regulates reconstruction of seminiferous tubule-like structure Reproductive biology, 2022-08-17;22(4):100684. 2022-08-17 [PMID: 35987158] (IHC, Western Blot, Mouse)
• A Po, A Citarella, G Catanzaro, ZM Besharat, S Trocchiane, F Gianno, C Sabato, M Moretti, E De Smaele, A Vacca, ME Fiori, E Ferretti Hedgehog-GLI signalling promotes chemoresistance through the regulation of ABC transporters in colorectal cancer cells Sci Rep, 2020-08-19;10(1):13988. 2020-08-19 [PMID: 32814794] (ICC, Human)
• X Lei, Y Zhong, L Huang, S Li, J Fu, L Zhang, Y Zhang, Q Deng, X Yu Identification of a novel tumor angiogenesis inhibitor targeting Shh/Gli1 signaling pathway in Non-small cell lung cancer Cell Death Dis, 2020-04-14;11(4):232. 2020-04-14 [PMID: 32286274] (IHC, Mouse)
• R Belgacemi, E Luczka, J Ancel, Z Diabasana, JM Perotin, A Germain, N Lalun, P Birembaut, X Dubernard, JC Mérol, G Delepine, M Polette, G Deslée, V Dormoy Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD EBioMedicine, 2019-12-23;51(0):102572. 12/23/2019 [PMID: 31877414]
• Dimitra Aravani, Gavin E. Morris, Peter D. Jones, Helena K. Tattersall, Elisavet Karamanavi, Michael A. Kaiser et al. HHIPL1 , a Gene at the 14q32 Coronary Artery Disease Locus, Positively Regulates Hedgehog Signaling and Promotes Atherosclerosis Circulation 8/6/2019 [PMID: 31163988]
• Dimitra Aravani, Gavin E. Morris, Peter D. Jones, Helena K. Tattersall, Elisavet Karamanavi, Michael A. Kaiser et al. HHIPL1 , a Gene at the 14q32 Coronary Artery Disease Locus, Positively Regulates Hedgehog Signaling and Promotes Atherosclerosis Circulation 2019-08-06 [PMID: 31163988] (Western Blot, Human)
• Amar N. Mirza, Siegen A. McKellar, Nicole M. Urman, Alexander S. Brown, Tyler Hollmig, Sumaira Z. Aasi et al. LAP2 Proteins Chaperone GLI1 Movement between the Lamina and Chromatin to Regulate Transcription Cell 1/1/2019 [PMID: 30503211]
• Camilla S Teng, Man-chun Ting, D'Juan T Farmer, Mia Brockop, Robert E Maxson, J Gage Crump Altered bone growth dynamics prefigure craniosynostosis in a zebrafish model of Saethre-Chotzen syndrome eLife 10/25/2018 [PMID: 30375332]
• Michael L. Drummond, Mischa Li, Eric Tarapore, Tuyen T.L. Nguyen, Baina J. Barouni, Shaun Cruz et al. Actin polymerization controls cilia-mediated signaling Journal of Cell Biology 9/3/2018 [PMID: 29945904]
• RJ Whitson, A Lee, NM Urman, A Mirza, CY Yao, AS Brown, JR Li, G Shankar, MA Fry, SX Atwood, EY Lee, ST Hollmig, SZ Aasi, KY Sarin, MP Scott, EH Epstein, JY Tang, AE Oro Noncanonical hedgehog pathway activation through SRF-MKL1 promotes drug resistance in basal cell carcinomas Nat. Med., 2018-02-05;0(0):. 2/5/2018 [PMID: 29400712]
• DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2017-11-13;15(1):39-46. 2017-11-13 [PMID: 29320487] (IHC, Mouse)
• Vitte J, Gao F, Coppola G et al. Timing of Smarcb1 and Nf2 inactivation determines schwannoma versus rhabdoid tumor development Nat Commun 8/21/2017 [PMID: 28824165]
• Mechanism of inhibition of the tumor suppressor Patched by Sonic Hedgehog Proc Natl Acad Sci USA, 2016-09-19;0(0):. 2016-09-19 [PMID: 27647915] (Western Blot, Mouse)
• Dong Zhou, Yingjian Li, Lili Zhou, Roderick J. Tan, Liangxiang Xiao, Min Liang et al. Sonic Hedgehog Is a Novel Tubule-Derived Growth Factor for Interstitial Fibroblasts after Kidney Injury Journal of the American Society of Nephrology 10/1/2014 [PMID: 24744439]
• Dong Zhou, Yingjian Li, Lili Zhou, Roderick J. Tan, Liangxiang Xiao, Min Liang et al. Sonic Hedgehog Is a Novel Tubule-Derived Growth Factor for Interstitial Fibroblasts after Kidney Injury Journal of the American Society of Nephrology 2014-10-01 [PMID: 24744439] (Western Blot, Human)
• Cigna N, Farrokhi Moshai E, Brayer S, Marchal-Somme J, Wemeau-Stervinou L, Fabre A, Mal H, Leseche G, Dehoux M, Soler P, Crestani B, Mailleux A The hedgehog system machinery controls transforming growth factor-beta-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis. Am J Pathol, 2012-09-29;181(6):2126-37. 2012-09-29 [PMID: 23031257] (IHC-P, Human)
• Strand MF, Wilson SR, Dembinski JL, Holsworth DD, Khvat A, Okun I, Petersen D, Krauss S A novel synthetic smoothened antagonist transiently inhibits pancreatic adenocarcinoma xenografts in a mouse model. PLoS ONE, 2011-06-15;6(6):e19904. 2011-06-15 [PMID: 21698280] (ICC, Mouse)

Reviews for GLI-1 Antibody (AF3455) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Mouse cell line.

reviewed by: Verified Customer WB Mouse cell line 12/08/2016

Summary

• Application: Western Blot
• Sample Tested: Cell lysate
• Species: Mouse cell line

reviewed by: Verified Customer IF Mouse 01/07/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 21698280
• Species: Mouse

Bioinformatics

• Gene Symbol: GLI1
• Uniprot:
  • P47806()