GITR Ligand/TNFSF18 Antibody [Biotin]

• Reactivity: Mu
• Applications: ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

GITR Ligand/TNFSF18 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse GITR Ligand/TNFSF18 (R&D Systems, Catalog # 2177-GL)
  Thr47-Ser173
  Accession # Q80YG2
• Specificity: Detects mouse GITR Ligand/TNFSF18 in ELISAs. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human GITR Ligand is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFSF18
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Mouse GITR Ligand/TNFSF18 Antibody (Catalog # MAB2177)
  ELISA Detection: Mouse GITR Ligand/TNFSF18 Biotinylated Antibody (Catalog # BAF2177)
  Standard: Recombinant Mouse GITR Ligand/TNFSF18 aa 47-173 (Catalog # 2177-GL)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GITR Ligand/TNFSF18 Antibody [Biotin]

• Activation-inducible TNF-related ligand
• AITRL
• AITRLMGC138237
• GITR Ligand
• GITRL
• GITRLglucocorticoid-induced TNFR-related protein ligand
• Glucocorticoid-induced TNF-related ligand
• hGITRLGITR ligand
• TL6AITR ligand
• TNFSF18
• tumor necrosis factor (ligand) superfamily, member 18
• tumor necrosis factor ligand superfamily member 18

Background

Glucocorticoid-induced TNF receptor superfamily-related protein ligand (GITRL) is a member of the TNF superfamily (TNFSF) and has been designated TNFSF18. Mouse GITRL cDNA encodes a 173 amino acid (aa) residues type II membrane protein with a C-terminal extracellular domain of 131 aa, an N-terminal cytoplasmic domain of 23 aa and a transmembrane domain of 19 aa. It shares approximately 60% aa sequence identity with human GITRL (2). Mouse GITRL is expressed at high levels in macrophages, dendritic cells and B cells. The expression is transiently up-regulated by LPS stimulation. GITRL binds to the type I transmembrane protein GITR/TNFRSF18, which is a member of the TNF receptor superfamily that is predominantly expressed on CD25⁺ regulatory CD4⁺ T cells (Treg). GITR is also expressed on naïve CD4⁺ CD25^- T cells, where its expression is up-regulated after antigen-driven activation (1, 2). Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2. GITRL provides co-stimulatory signals for activated CD4⁺ CD25^- T cells to enhance cell proliferation and augment cytokine production. On CD4⁺ CD25⁺ Treg cells, GITRL also provides co-stimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state that reverses the suppressive function of Treg cells. GITRL-GITR ligation provides important co-stimulatory signals that play important roles in modulating diverse T cell functions (1-4).

1. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
2. Ji, H. et al. (2004) J. Immunology 172:5823.
3. Kanamaru, F. et al. (2004) J. Immunology 172:7306.
4. Ronchetti, S. et al. (2004) Eur. J. Immunology 34:613.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GITR Ligand/TNFSF18 Antibody (BAF2177)(1)

Publication using BAF2177

• La Cava A, Ebling FM, Hahn BH Ig-reactive CD4+CD25+ T cells from tolerized (New Zealand Black x New Zealand White)F1 mice suppress in vitro production of antibodies to DNA. J. Immunol., 2004-09-01;173(5):3542-8. 2004-09-01 [PMID: 15322219] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TNFSF18
• Uniprot:
  • Q80YG2()