GITR Ligand/TNFSF18 Antibody (994529) [Unconjugated]

NS0 cell line transfected with either mouse GITR Ligand/TNFRSF18 (filled histogram) or irrelevant protein (open histogram) was stained with Rat Anti-Mouse GITR Ligand/TNFRSF18 Monoclonal Antibody (Catalog # MAB21773) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

In the presence of Rat Anti-Mouse CD3 Monoclonal Anti-body (0.5 µg/mL, MAB4841) and a cross-linking antibody, Mouse Anti-His Tag Monoclonal Antibody (10 µg/mL, MAB050), Recombinant Mouse GITR Ligand/TNFSF18 (2177-GL) stimulates proliferation in mouse CD4+T cells in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Under these conditions, proliferation elicited by Recombinant Mouse GITR Ligand/TNFSF18 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GITR Ligand/ TNFSF18 Monoclonal Antibody (Catalog # MAB21773). The ND50 is typically 1.2-12 µg/mL.

• Reactivity: Mu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 994529
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

GITR Ligand/TNFSF18 Antibody (994529) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse GITR Ligand/TNFSF18
  Thr47-Ser173
  Accession # Q7TS55
• Specificity: Detects mouse GITR Ligand/TNFSF18 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 1.2-12 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GITR Ligand/TNFSF18 Antibody (994529) [Unconjugated]

• Activation-inducible TNF-related ligand
• AITRL
• AITRLMGC138237
• GITR Ligand
• GITRL
• GITRLglucocorticoid-induced TNFR-related protein ligand
• Glucocorticoid-induced TNF-related ligand
• hGITRLGITR ligand
• TL6AITR ligand
• TNFSF18
• tumor necrosis factor (ligand) superfamily, member 18
• tumor necrosis factor ligand superfamily member 18

Background

Glucocorticoid-induced TNF receptor superfamily-related protein ligand (GITRL) is a member of the TNF superfamily (TNFSF) and has been designated TNFSF18. Mouse GITRL cDNA encodes a 173 amino acid (aa) type II membrane protein with a C-terminal extracellular domain of 131 aa, an N-terminal cytoplasmic domain of 23 aa and a transmembrane domain of 19 aa. It shares approximately 60% aa sequence identity with human GITRL (2). Mouse GITRL is expressed at high levels in macrophages, dendritic cells and B cells. The expression is transiently upregulated by LPS stimulation. GITRL binds to the type I transmembrane protein GITR/TNFRSF18, which is a member of the TNF receptor superfamily that is predominantly expressed on CD25⁺ regulatory CD4⁺ T cells (Treg). GITR is also expressed on naïve CD4⁺ CD25^- T cells, where its expression is upregulated after antigen-driven activation (1, 2). Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2. GITRL provides costimulatory signals for activated CD4⁺ CD25^- T cells to enhance cell proliferation and augment cytokine production. On CD4⁺ CD25⁺ Treg cells, GITRL also provides costimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state that reverses the suppressive function of Treg cells. GITRL-GITR ligation provides important costimulatory signals that play important roles in modulating diverse T cell functions (1-4).

1. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
2. Ji, H. et al. (2004) J. Immunology 172:5823.
3. Kanamaru, F. et al. (2004) J. Immunology 172:7306.
4. Ronchetti, S. et al. (2004) Eur. J. Immunology 34:613.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q7TS55()