GITR Ligand/TNFSF18 Antibody (994529) [Alexa Fluor® 405]

• Reactivity: Mu
• Applications: Flow
• Clone: 994529
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 405

GITR Ligand/TNFSF18 Antibody (994529) [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse GITR Ligand/TNFSF18
  Thr47-Ser173
  Accession # Q7TS55
• Specificity: Detects mouse GITR Ligand/TNFSF18 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Reconstitution Instructions: Reconstitution default not known

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GITR Ligand/TNFSF18 Antibody (994529) [Alexa Fluor® 405]

• Activation-inducible TNF-related ligand
• AITRL
• AITRLMGC138237
• GITR Ligand
• GITRL
• GITRLglucocorticoid-induced TNFR-related protein ligand
• Glucocorticoid-induced TNF-related ligand
• hGITRLGITR ligand
• TL6AITR ligand
• TNFSF18
• tumor necrosis factor (ligand) superfamily, member 18
• tumor necrosis factor ligand superfamily member 18

Background

Glucocorticoid-induced TNF receptor superfamily-related protein ligand (GITRL) is a member of the TNF superfamily (TNFSF) and has been designated TNFSF18. Mouse GITRL cDNA encodes a 173 amino acid (aa) type II membrane protein with a C-terminal extracellular domain of 131 aa, an N-terminal cytoplasmic domain of 23 aa and a transmembrane domain of 19 aa. It shares approximately 60% aa sequence identity with human GITRL (2). Mouse GITRL is expressed at high levels in macrophages, dendritic cells and B cells. The expression is transiently upregulated by LPS stimulation. GITRL binds to the type I transmembrane protein GITR/TNFRSF18, which is a member of the TNF receptor superfamily that is predominantly expressed on CD25⁺ regulatory CD4⁺ T cells (Treg). GITR is also expressed on naïve CD4⁺ CD25^- T cells, where its expression is upregulated after antigen-driven activation (1, 2). Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2. GITRL provides costimulatory signals for activated CD4⁺ CD25^- T cells to enhance cell proliferation and augment cytokine production. On CD4⁺ CD25⁺ Treg cells, GITRL also provides costimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state that reverses the suppressive function of Treg cells. GITRL-GITR ligation provides important costimulatory signals that play important roles in modulating diverse T cell functions (1-4).

1. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
2. Ji, H. et al. (2004) J. Immunology 172:5823.
3. Kanamaru, F. et al. (2004) J. Immunology 172:7306.
4. Ronchetti, S. et al. (2004) Eur. J. Immunology 34:613.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q7TS55()