GITR Ligand/TNFSF18 Antibody (337122) [Unconjugated]

In the presence of Mouse CD3 epsilon Monoclonal Anti­body (0.5 µg/mL, Catalog # MAB484) and a cross-linking antibody, polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Mouse GITR Ligand/TNFSF18 (Catalog # 2177-GL) stimulates proliferation in mouse CD4+ T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Mouse GITR Ligand/TNFSF18 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GITR Ligand/TNFSF18 Monoclonal Antibody (Catalog # MAB2177). The ND50 is typically 2-8 µg/mL.

• Reactivity: Mu
• Applications: B/N, ELISA(Cap)
• Clone: 337122
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

GITR Ligand/TNFSF18 Antibody (337122) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse GITR Ligand/TNFSF18
  Thr47-Ser173
  Accession # Q80YG2
• Specificity: Detects mouse GITR Ligand/TNFSF18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human GITR Ligand, recombinant mouse (rm) CD40, or rmTNF-alpha is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TNFSF18
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 2-8 ug/mL
• Application Notes: ELISA Detection: Mouse GITR Ligand/TNFSF18 Biotinylated Antibody (Catalog number BAF2177) Standard: Recombinant Mouse GITR Ligand/TNFSF18 aa 47-173 (Catalog number 2177-GL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GITR Ligand/TNFSF18 Antibody (337122) [Unconjugated]

• Activation-inducible TNF-related ligand
• AITRL
• AITRLMGC138237
• GITR Ligand
• GITRL
• GITRLglucocorticoid-induced TNFR-related protein ligand
• Glucocorticoid-induced TNF-related ligand
• hGITRLGITR ligand
• TL6AITR ligand
• TNFSF18
• tumor necrosis factor (ligand) superfamily, member 18
• tumor necrosis factor ligand superfamily member 18

Background

Glucocorticoid-induced TNF receptor superfamily-related protein ligand (GITRL) is a member of the TNF superfamily (TNFSF) and has been designated TNFSF18. Mouse GITRL cDNA encodes a 173 amino acid (aa) residues type II membrane protein with a C-terminal extracellular domain of 131 aa, an N-terminal cytoplasmic domain of 23 aa and a transmembrane domain of 19 aa. It shares approximately 60% aa sequence identity with human GITRL (2). Mouse GITRL is expressed at high levels in macrophages, dendritic cells and B cells. The expression is transiently up-regulated by LPS stimulation. GITRL binds to the type I transmembrane protein GITR/TNFRSF18, which is a member of the TNF receptor superfamily that is predominantly expressed on CD25 + regulatory CD4 + T cells (Treg). GITR is also expressed on naïve CD4 + CD25 - T cells, where its expression is up-regulated after antigen-driven activation (1, 2). Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2. GITRL provides co-stimulatory signals for activated CD4 + CD25 - T cells to enhance cell proliferation and augment cytokine production. On CD4 + CD25 + Treg cells, GITRL also provides co-stimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state that reverses the suppressive function of Treg cells. GITRL-GITR ligation provides important co-stimulatory signals that play important roles in modulating diverse T cell functions (1-4).

1. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
2. Ji, H. et al. (2004) J. Immunology 172:5823.
3. Kanamaru, F. et al. (2004) J. Immunology 172:7306.
4. Ronchetti, S. et al. (2004) Eur. J. Immunology 34:613.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GITR Ligand/TNFSF18 Antibody (MAB2177)(3)

Publications using MAB2177

• Bharani Thangavelu, Angela M. Boutté Single Molecule Assay for Ultrasensitive Detection of Cathepsin B in Human Blood ACS Omega 2021-04-13 [PMID: 33869941] (ELISA Capture, Human)
• Bharani Thangavelu, Angela M. Boutté Single Molecule Assay for Ultrasensitive Detection of Cathepsin B in Human Blood ACS Omega 4/13/2021 [PMID: 33869941]
• Mahmud , Shawn A, Manlove , Luke S, Schmitz , Heather, Xing , Yan, Wang , Yanyan, Owen , David L, Schenkel , Jason M, Boomer , Jonathan, Green , Jonathan, Yagita , Hideo, Chi , Hongbo, Hogquist , Kristin, Farrar , Michael Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells. Nat Immunol, 2014-03-16;15(5):473-81. 2014-03-16 [PMID: 24633226] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: TNFSF18
• Uniprot:
  • Q80YG2()