GITR Ligand/TNFSF18 Antibody (109101) [PerCP (Peridinin-chlorophyll Protein Complex)]

• Applications: Flow
• Clone: 109101
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PerCP (Peridinin-chlorophyll Protein Complex)

GITR Ligand/TNFSF18 Antibody (109101) [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GITR Ligand/TNFSF18
  Glu52-Ser177
  Accession # Q9UNG2
• Specificity: Detects human GITR Ligand/TNFSF18.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF18
• Purity Statement: Protein A or G purified from ascites

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

GITR (Glucocorticoid-Induced TNF Receptor superfamily-related protein, also named AITR, Activation-Inducible TNF Receptor superfamily-related protein) and GITR ligand (GITRL) are novel members of the TNF receptor (TNFR) and TNF superfamilies (SF) that have been designated TNFRSF18 and TNFSF18, respectively. Human GITRL cDNA encodes a 177 amino acid residues type II membrane protein. The carboxy-terminal extracellular domain shows sequence identity to TNF/TNFSF2 (21%), Fas ligand/TNFSF6 (21%), TRAIL/TNFSF10 (18%), and lymphotoxin alpha /TNFSF1 (18%). GITRL is constitutively expressed in human umbilical vein endothelial cells but is not expressed in resting or stimulated T cell lines, B cell lines or peripheral blood mononuclear cells. GITR, the receptor for GITRL, is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up-regulated by antigen-receptor stimulation or by treatment with either soluble anti-CD3 plus anti-CD28, or PMA plus ionomycin. Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2 and protect cells from TCR activation-induced cell death. It has been proposed that GITRL and GITR may modulate T lymphocyte functions in peripheral tissues.

1. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
2. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
3. Gurney, A.L. et al. (1999) Curr. Biol. 9:215.
4. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFSF18