GIT1 Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: GIT1 Antibody [NBP3-03734] - Analysis of U-2 OS cells using GIT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

ICC/IF-GIT1 Antibody [NBP3-03734] - Analysis of L929 cells using GIT1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: GIT1 Antibody - Azide and BSA Free [NBP3-03734] - Immunofluorescence analysis of C6 cells using [KO Validated] GIT1 Rabbit pAb (A15437) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western Blot: GIT1 Antibody [NBP3-03734] - Analysis of extracts of various cell lines, using GIT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Western Blot: GIT1 Antibody [NBP3-03734] - Analysis of extracts from normal (control) and GIT1 knockout (KO) HeLa cells, using GIT1 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GIT1 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 460-640 of human GIT1 (NP_054749.2). EIHKLQAENLQLRQPPGPVPTPPLPSERAEHTPMAPGGSTHRRDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFTPSSPLLSCSQEGSRHTSKLSRHGSGADSDYENTQSGDPLLGLEGKRFLELGKEEDFHPELESLDGDLDPGLP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GIT1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Knockout Validated
  • Western Blot 1:200-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for GIT1 Antibody - Azide and BSA Free

• ARF GAP GIT1
• ARF GTPase-activating protein GIT1
• CAT1
• CAT-1
• Cool-associated and tyrosine-phosphorylated protein 1
• G protein-coupled receptor kinase interacting ArfGAP 1
• G protein-coupled receptor kinase interactor 1
• G protein-coupled receptor kinase-interactor 1
• GIT1
• GRK-interacting protein 1

Background

G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules. Alternative names for GIT1 include GRK-interacting protein 1, cool-associated and tyrosine phosphorylated protein 1, and Cat-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: GIT1