Girdin [p Tyr1764] Antibody

Western Blot: Girdin [p Tyr1764] Antibody [NBP3-23352] - Western blot image of human A431 cell lysates treated with pervanadate (lanes 1-4). The blot was treated with alkaline phosphatase to dephosphorylate Girdin phosphosites (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Girdin (lanes 1 & 2) or rabbit polyclonal anti-Girdin (Tyr-1764), phospho-specific (lanes 3 & 4).

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Girdin [p Tyr1764] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-Girdin (Tyr-1764) synthetic peptide (coupled to carrier) corresponding to amino acid residues around tyrosine 1764 in human Girdin. This sequence has high homology to rat and mouse Girdin, and the site is not conserved in other Girdin family members. [UniProt# Q3V6T2]
• Modification: p Tyr1764
• Specificity: The antibody was cross-absorbed to unphosphorylated Girdin (Tyr-1764) peptide before affinity purification using phospho-Girdin (Tyr-1764) peptide. This antibody detects a 250 kDa protein on SDS-PAGE immunoblots of human A431 cells stimulated with EGF and Pervanadate. These reactivities are not observed after alkaline phosphatase treatment to dephosphorylate Girdin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCDC88A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 250 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Girdin [p Tyr1764] Antibody

• Akt phosphorylation enhancer
• APE
• APEGIRDIN
• CCDC88A
• coiled-coil domain containing 88A
• Coiled-coil domain-containing protein 88A
• G alpha-interacting vesicle-associated protein
• G{alpha}-interacting vesicle-associated protein
• Girders of actin filament
• Girdin
• GIV
• GIVFLJ10392
• GRDN
• GRDNAKT-phosphorylation enhancer
• HkRP1
• HkRP1Galpha-interacting vesicle-associated protein
• Hook-related protein 1
• KIAA1212DKFZp686D0630

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCDC88A