GilZ Knockout HeLa Cell Lysate


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Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

GilZ Knockout HeLa Cell Lysate Summary

Knockout achieved by using CRISPR/Cas9,77 bp deletion in exon 3 and Insertion of the selection cassette in exon 3 and Insertion of the selection cassette in exon 3


Application Notes
You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
Reconstitution Instructions
To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

Knockout HeLa Cell


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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for GilZ Knockout HeLa Cell Lysate

  • Delta sleep-inducing peptide immunoreactor
  • DSIP-immunoreactive leucine zipper protein
  • DSIP-immunoreactive peptide
  • GILZDKFZp313A1123
  • Glucocorticoid-induced leucine zipper protein
  • Protein DIP
  • TSC22 domain family protein 3
  • TSC22 domain family, member 3
  • TSC-22 related protein
  • TSC-22-like protein
  • TSC-22-related protein
  • TSC-22Rimmunoreactor


This gene encodes the anti-inflammatory protein glucocorticoid (GC)-induced leucine zipper. Expression of this gene stimulated by glucocorticoids and interleukin 10 and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid. This protein has also been shown to inhibit pro-inflammatory molecules including nuclear factor \&\#954;B. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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Gene Symbol TSC22D3