GilZ Antibody (CFMKG15) - BSA Free

Western Blot: GilZ Antibody (CFMKG15) [NBP2-00143] - Left: Mouse thymocytes cultured without (lane 1) or with (lane 2) dexamethasone (10e-7 M) for 3 hr. The membrane was probed with 10 ug/mL of Anti-Human/Mouse GILZ Purified, followed by Anti-Rat IgG HRP. Right: Normal human peripheral blood cells cultured without (lane 1) or with (lane 2) dexamethasone (10e-7 M) for 3 hours. The membrane was probed with 2 ug/mL of Anti-Human/Mouse GILZ Purified, followed by Anti-Rat IgG HRP.

Flow Cytometry: GilZ Antibody (CFMKG15) [NBP2-00143] - Analysis using the PE conjugate of NBP2-00143. C57BL/6 lymph node cells were cultured for 3 hr with (purple histogram) or without (blue histogram) dexamethasone, followed by intracellular staining with 0.25 ug of Rat IgG2a kappa Isotype Control PE (filled histogram) or 0.25 ug of Anti-Human/Mouse GILZ PE (filled histograms). CD3+ cells in the lymphocyte gate were used for analysis.

• Reactivity: Mu
• Applications: WB, Flow
• Clone: CFMKG15
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

GilZ Antibody (CFMKG15) - BSA Free Summary

• Description: Novus Biologicals Rat GilZ Antibody (CFMKG15) - BSA Free (NBP2-00143) is a monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody is GILZ.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: TSC22D3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Western Blot 1:100-1:2000
• Application Notes: This CFMKG15 antibody has been tested by western blot analysis of dexamethasone-treated mouse thymocytes. CFMKG15 recognizes endogenous mouse GILZ with an approximate molecular weight of 14 kDa; a non-specific band of approximately 95 kDa is also observed. CFMKG15 also recognizes endogenous human GILZ in dexamethasone-treated peripheral blood mononuclear cells, with an approximate molecular weight of 14 kDa; non-specific bands may also appear at approximately 33 kDa and 95 kDa. This can be used at less than or equal to 2 ug/mL. This CFMKG15 antibody has also been reported for use in immunocytochemistry. (Fluorochrome conjugated CFMKG15 is recommended for use in intracellular flow cytometry) It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for GilZ Antibody (CFMKG15) - BSA Free

• Delta sleep-inducing peptide immunoreactor
• DSIP-immunoreactive leucine zipper protein
• DSIP-immunoreactive peptide
• GILZDKFZp313A1123
• Glucocorticoid-induced leucine zipper protein
• Protein DIP
• TSC22 domain family protein 3
• TSC22 domain family, member 3
• TSC-22 related protein
• TSC-22-like protein
• TSC-22-related protein
• TSC-22Rimmunoreactor

Background

GILZ is a leucine-zipper-containing protein that is induced by glucocorticoids. It is expressed in mast cells, monocytes, macrophages, dendritic cells, and T cells. GILZ has been shown to inhibit production of IL-2 as well as TCR-driven upregulation of FasL. GILZ mediates these changes in transcription, at least in part, by inhibiting DNA-binding of the transcription factors AP-1 and NF-kB. GILZ has also been shown to mediate antiinflammatory signals in macrophages and dendritic cells by interfering with NF-kB-mediated signaling. Although GILZ has been reported to localize to the nucleus, more recent reports as well as testing have observed predominantly cytoplasmic staining.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TSC22D3