Human Ghrelin ELISA Kit (Colorimetric)

ELISA: Human Ghrelin ELISA Kit (Colorimetric) [NBP2-66709] - Samples were spiked with high concentrations of Human Ghrelin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Ghrelin ELISA Kit (Colorimetric) [NBP2-66709] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Milk, Cell Supernatant, Tissue Homogenate
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Human Ghrelin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes the acylated version of Human GHRL in samples. No significant cross-reactivity or interference between Human GHRL and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.08%
• Intra-Assay: CV% < 5.78%
• Spike Recovery: 83-100%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GHRL

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Ghrelin ELISA Kit (Colorimetric)

• appetite-regulating hormone
• Ghrelin
• ghrelin/obestatin preprohormone
• ghrelin/obestatin prepropeptide
• GHRL
• Growth hormone secretagogue
• Growth hormone-releasing peptide
• Motilin-related Peptide
• MTLRPgrowth hormone secretagogue receptor ligand
• obestatin
• Protein M46

Background

Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Ghrelin ELISA Kit (NBP2-66709)(1)

Publication using NBP2-66709

• Tuccinardi D, Di Mauro A, Lattanzi G Et al. An Extra Virgin Olive Oil-enriched Chocolate Spread Positively Modulates Insulin-Resistance Markers compared With a Palm Oil-enriched one in Healthy Young Adults: A Double-Blind, Cross-Over, Randomized Controlled Trial Diabetes/metabolism research and reviews 2021-08-25 [PMID: 34435429] (ELISA)

FAQs for Ghrelin ELISA Kit (NBP2-66709). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GHRL