Human, Mouse, Rat Ghrelin ELISA Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1863
    • Availability
      Product Discontinued

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Human, Mouse, Rat Ghrelin ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard Curve. Ghrelin (Human Mouse Rat) ELISA Kit is an in vitro quantitative assay for detecting Ghrelin based on the principle of Competitive Enzyme Immunoassay.
Standard Curve Range
0.1 to 1000 ng/mL
Sensitivity
161 pg/mL
Assay Type
Competitive ELISA
Inter-Assay
CV% < 15%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GHRL

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Useful for quantification.
Reviewed Applications
Read 1 Review rated 4
using
KA1863 in the following application:

Publications
Read Publication using KA1863.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. *Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
  2. 5X Assay Diluent B (Item E): 5X concentrated buffer. Diluent for both standardsand samples including serum, plasma, cell culture media or other sampletypes.
  3. Anti-Ghrelin Polyclonal Antibody (Item N): Lyophilized anti-Ghrelin.
  4. Biotinylated Ghrelin Peptide (Item F): Lyophilized Biotinylated Ghrelin Peptide,1 vial is enough to assay the whole plate.
  5. EIA Microplate (Item A): 96 wells (12 strips x 8 wells) coated with secondaryantibody. *
  6. EIA Wash Buffer Concentrate (20x) (Item B): 20X concentrated solution.
  7. HRP-Streptavidin Concentrate (Item G): 50X concentrated HRP-conjugatedstreptavidin.
  8. Positive Control (Item M): Lyophilized Positive Control.
  9. Standard Ghrelin Peptide (Item C): Lyophilized Ghrelin Peptide. 1 vial isenough to run each standard in duplicate.
  10. Stop Solution (Item I): 0.2 M sulfuric acid.
  11. TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’- tetramethylbenzidine(TMB) in buffered solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human, Mouse, Rat Ghrelin ELISA Kit (Colorimetric)

  • appetite-regulating hormone
  • Ghrelin
  • ghrelin/obestatin preprohormone
  • ghrelin/obestatin prepropeptide
  • GHRL
  • Growth hormone secretagogue
  • Growth hormone-releasing peptide
  • Motilin-related Peptide
  • MTLRPgrowth hormone secretagogue receptor ligand
  • obestatin
  • Protein M46

Background

Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Ghrelin ELISA Kit (KA1863)(1)

Review for Ghrelin ELISA Kit (KA1863) (1) 41

Average Rating: 4
(Based on 1 review)

Reviews using KA1863:
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 Ghrelin KA1863
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Verified Customer
10/11/2019
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Summary

Sample TestedEDTA Plasma
Lot0514197010

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Ghrelin ELISA Kit (KA1863). (Showing 1 - 2 of 2 FAQ).

  1. Do you know if this ghrelin ELISA kit distinguishes between acylated ghrelin and de-acylated ghrelin?
    • This kit has not been tested on the different forms of Ghrelin. It recognizes AA 39-51 of human Ghrelin. The lab thinks it will likely be able to detect both forms
  2. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Additional Ghrelin Products

Blogs on Ghrelin.

Ghrelin: Targeting the Hunger Hormone to Combat Obesity and Type 2 Diabetes
As the hormone ghrelin is linked to appetite and weight gain, as well as impaired glucose-induced insulin secretion, there is considerable interest in this peptide as a potential drug target. Although the overall lack of success in this field has been...  Read full blog post.

Shades of Ghrelin in Weight Homeostasis
The neuropeptide and gut hormone Ghrelin is an endogenous ligand for the growth hormone (GH)-secretagogue receptor (GHSR) within the central nervous system. This pathway has received a great deal of attention and heavy study within the last decade bec...  Read full blog post.

Appetite and Energy: A Ghrelin Balancing Act
Ghrelin is the only potent orexigenic peptide in circulation. It stimulates food intake and leads to metabolism regulation, positive energy balance, adipogenesis, and body weight gain. However, in studies using ghrelin antibodies, the physiological si...  Read full blog post.

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Verified Customer
10/11/2019
Application:
Species:

Bioinformatics

Gene Symbol GHRL