Human, Mouse, Rat Ghrelin ELISA Kit (Colorimetric)

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ELISA: Ghrelin/Obestatin ELISA Kit [Biotin] [KA1863] - Standard curve

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum
Standard Curve Range
0.1 to 1000 ng/mL
Sensitivity
161 pg/mL

Order Details

Human, Mouse, Rat Ghrelin ELISA Kit (Colorimetric) Summary

Description
Ghrelin (Human Mouse Rat) ELISA Kit is an in vitro quantitative assay for detecting Ghrelin based on the principle of Competitive Enzyme Immunoassay.
Standard Curve Range
0.1 to 1000 ng/mL
Sensitivity
161 pg/mL
Assay Type
Competitive ELISA
Inter-Assay
CV% < 15%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GHRL

Applications/Dilutions

Application Notes
Useful for quantification.
Publications
Read Publication using KA1863.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’- tetramethylbenzidine(TMB) in buffered solution.
  2. Standard Ghrelin Peptide (Item C): Lyophilized Ghrelin Peptide. 1 vial isenough to run each standard in duplicate.
  3. Stop Solution (Item I): 0.2 M sulfuric acid.
  4. 5X Assay Diluent B (Item E): 5X concentrated buffer. Diluent for both standardsand samples including serum, plasma, cell culture media or other sampletypes.
  5. Anti-Ghrelin Polyclonal Antibody (Item N): Lyophilized anti-Ghrelin.
  6. *Return unused wells to the pouch containing desiccant pack, reseal along entire edge.
  7. HRP-Streptavidin Concentrate (Item G): 50X concentrated HRP-conjugatedstreptavidin.
  8. Positive Control (Item M): Lyophilized Positive Control.
  9. Biotinylated Ghrelin Peptide (Item F): Lyophilized Biotinylated Ghrelin Peptide,1 vial is enough to assay the whole plate.
  10. EIA Microplate (Item A): 96 wells (12 strips x 8 wells) coated with secondaryantibody. *
  11. EIA Wash Buffer Concentrate (20x) (Item B): 20X concentrated solution.

Notes

Quality control test: Standard Curve.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human, Mouse, Rat Ghrelin ELISA Kit (Colorimetric)

  • appetite-regulating hormone
  • Ghrelin
  • ghrelin/obestatin preprohormone
  • ghrelin/obestatin prepropeptide
  • GHRL
  • Growth hormone secretagogue
  • Growth hormone-releasing peptide
  • Motilin-related Peptide
  • MTLRPgrowth hormone secretagogue receptor ligand
  • obestatin
  • Protein M46

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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KA1863
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Applications: ELISA

Publications for Ghrelin ELISA Kit (KA1863)(1)

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Product General Protocols

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FAQs for Ghrelin ELISA Kit (KA1863) (0)

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Blogs on Ghrelin.

Ghrelin: Targeting the Hunger Hormone to Combat Obesity and Type 2 Diabetes
As the hormone ghrelin is linked to appetite and weight gain, as well as impaired glucose-induced insulin secretion, there is considerable interest in this peptide as a potential drug target. Although the overall lack of success in this field has been...  Read full blog post.

Shades of Ghrelin in Weight Homeostasis
The neuropeptide and gut hormone Ghrelin is an endogenous ligand for the growth hormone (GH)-secretagogue receptor (GHSR) within the central nervous system. This pathway has received a great deal of attention and heavy study within the last decade bec...  Read full blog post.

Appetite and Energy: A Ghrelin Balancing Act
Ghrelin is the only potent orexigenic peptide in circulation. It stimulates food intake and leads to metabolism regulation, positive energy balance, adipogenesis, and body weight gain. However, in studies using ghrelin antibodies, the physiological si...  Read full blog post.

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Bioinformatics

Gene Symbol GHRL
Entrez