GGA3 Antibody

Immunohistochemistry-Paraffin: GGA3 Antibody [NB100-2444] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Rt, Ca, Mu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GGA3 Antibody Summary

• Immunogen: Peptide with sequence C-EQLSTEVGEVDQ corresponding to C-Terminus according to NP_619525.1, NP_054720.1.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_619525 and NP_054720)
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GGA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Preliminary experiments gave an approx. 65 kDa band in mouse and rat brain lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.0 kDa band according to NP_766636.2 and 70.2 kDa band according to NP_001238996.1. The 65 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GGA3 Antibody

• ADP-ribosylation factor-binding protein GGA3
• golgi associated, gamma adaptin ear containing, ARF binding protein 3
• golgi-associated, gamma adaptin ear containing, ARF binding protein 3
• KIAA0154Golgi-localized, gamma ear-containing, ARF-binding protein 3

Background

GGA3 encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network (TGN) and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma- adaptins. GGA3 mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Alternative splicing of this gene results in two transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GGA3 Antibody (NB100-2444)(1)

Publication using NB100-2444

• Prag G, Lee S, Mattera R et al. Structural mechanism for ubiquitinated-cargo recognition by the Golgi-localized, gamma-ear-containing, ADP-ribosylation-factor-binding proteins. Proc Natl Acad Sci U S A 2005-02-15 [PMID: 15701688]

Bioinformatics

• Gene Symbol: GGA3
• Entrez:
  • 23163(Human)
• Uniprot:
  • NP_619525.1(Human)