Gemin 5 Antibody - Azide and BSA Free

Western Blot: Gemin 5 Antibody [NBP3-15523] - Western blot analysis of extracts of HeLa cells, using Gemin 5 Rabbit pAb (NBP3-15523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry-Paraffin: Gemin 5 Antibody [NBP3-15523] - Immunohistochemistry of paraffin-embedded rat kidney using Gemin 5 antibody (NBP3-15523) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Gemin 5 Antibody [NBP3-15523] - Immunohistochemistry of paraffin-embedded human placenta using Gemin 5 antibody (NBP3-15523) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Gemin 5 Antibody [NBP3-15523] - Immunohistochemistry of paraffin-embedded rat heart using Gemin 5 antibody (NBP3-15523) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Gemin 5 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Gemin 5 Antibody - Azide and BSA Free (NBP3-15523) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 750-950 of human Gemin 5 (NP_056280.2). RTPVKLESIDGNEEESMKENSGPVENGVSDQEGEEQAREPELPCGLAPAVSREPVICTPVSSGFEKSKVTINNKVILLKKEPPKEKPETLIKKRKARSLLPLSTSLDHRSKEELHQDCLVLATAKHSRELNEDVSADVEERFHLGLFTDRATLYRMIDIEGKGHLENGHPELFHQLMLWKGDLKGVLQTAAERGELTDNLV
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GEMIN5
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for Gemin 5 Antibody - Azide and BSA Free

• DKFZp586M1824
• EC 2.7.7.8
• EC 5.4.2.1
• gem (nuclear organelle) associated protein 5
• gem-associated protein 5
• gemin 5
• Gemin5
• MGC142174

Background

GEMIN5 is part of a large macromolecular complex localized to both the cytoplasm and the nucleus that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN (MIM 600354), GEMIN2 (SIP1; MIM 602595), GEMIN3 (DDX20; MIM 606168), and GEMIN4 (MIM 606969).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: GEMIN5