Gemin 5 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Gemin 5 Antibody [NBP1-90354] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies & cytosol.

Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.

Staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Staining of human skin shows strong cytoplasmic positivity in squamous epithelial cells.

Analysis in human cell line CACO-2.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Gemin 5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Gemin 5 Antibody - BSA Free (NBP1-90354) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: YDSGSFTIMQEVYSAFLPDGCDHLRDKLGDHQSPATPAFKSLEAFFLYGRLYEFWWSLSRPCPNSSVWVRAGHRTLSVEPSQQLDTASTEETDPET
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GEMIN5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Gemin 5 Protein (NBP1-90354PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Gemin 5 Antibody - BSA Free

• DKFZp586M1824
• EC 2.7.7.8
• EC 5.4.2.1
• gem (nuclear organelle) associated protein 5
• gem-associated protein 5
• gemin 5
• Gemin5
• MGC142174

Background

GEMIN5 is part of a large macromolecular complex localized to both the cytoplasm and the nucleus that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN (MIM 600354), GEMIN2 (SIP1; MIM 602595), GEMIN3 (DDX20; MIM 606168), and GEMIN4 (MIM 606969).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GEMIN5