GDNF Antibody

Western Blot: GDNF Antibody [H00002668-B01P] - GDNF in rat tissue with pAb dilution of 1:1000. This image was submitted via customer Review.

Western Blot: GDNF Antibody [H00002668-B01P] - Analysis of GDNF expression in transfected 293T cell line by GDNF polyclonal antibody. Lane 1: GDNF transfected lysate(23.21 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

GDNF Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: GDNF (NP_000505.1, 1 a.a. - 211 a.a.) full-length human protein. MKLWDVVAVCLVLLHTASAFPLPAGKRPPEAPAEDRSLGRRRAPFALSSDSNMPEDYPDQFDDVMDFIQATIKRLKRSPDKQMAVLPRRERNRQAAAANPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI
• Specificity: GDNF - glial cell derived neurotrophic factor,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: GDNF
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GDNF Antibody

• Astrocyte-derived trophic factor
• ATF
• ATF1
• ATF2
• GDNF
• glial cell derived neurotrophic factor
• glial cell line derived neurotrophic factor
• glial cell line-derived neurotrophic factor
• HFB1-GDNF
• HGDNF
• HSCR3

Background

This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for GDNF Antibody (H00002668-B01P) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 07/29/2017

Summary

• Application: Western Blot
• Sample Tested: rat tissue
• Species: Rat

Comments

• Comments: GDNFantibody (1 : 1000) was used in Western Blot.it work well.

Bioinformatics

• Gene Symbol: GDNF
• Entrez:
  • 2668(Human)
• Uniprot:
  • NP_000505.1(Human)