GDNF Antibody [Unconjugated]

Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH-SY5Y human neuroblastoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by GDNF (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AB-212-NA). The ND50is typically 7.5-30 µg/mL.

• Reactivity: Hu, Rt
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

GDNF Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human GDNF
  Arg109-Ile211
  Accession # P39905
• Specificity: Detects human and rat GDNF in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GDNF
• Purity Statement: Protein A or G purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 7.5-30 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDNF Antibody [Unconjugated]

• Astrocyte-derived trophic factor
• ATF
• ATF1
• ATF2
• GDNF
• glial cell derived neurotrophic factor
• glial cell line derived neurotrophic factor
• glial cell line-derived neurotrophic factor
• HFB1-GDNF
• HGDNF
• HSCR3

Background

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GDNF Antibody (AB-212-NA)(4)

Publications using AB-212-NA

• CW Gantner, IR de Luzy, JA Kauhausen, N Moriarty, JC Niclis, CR Bye, V Penna, CPJ Hunt, CM Ermine, CW Pouton, D Kirik, LH Thompson, CL Parish Viral Delivery of GDNF Promotes Functional Integration of Human Stem Cell Grafts in Parkinson&#039;s Disease Cell Stem Cell, 2020-02-13;0(0):. 2020-02-13 [PMID: 32059808] (IHC, Mouse, Rat)
• S Nencini, M Ringuet, DH Kim, C Greenhill, JJ Ivanusic Gdnf, Neurturin and Artemin Activate and Sensitize Bone Afferent Neurons and Contribute To Inflammatory Bone Pain J. Neurosci., 2018-04-30;0(0):. 2018-04-30 [PMID: 29712778] (Neutralization, Rat)
• Pia Runeberg-Roos, Elisa Piccinini, Anna-Maija Penttinen, Kert Mätlik, Hanna Heikkinen, Satu Kuure et al. Developing therapeutically more efficient Neurturin variants for treatment of Parkinson's disease Neurobiology of Disease 2016-12-01 [PMID: 27425888]
• Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence Nature, 2016-07-13;535(7612):440-3. 2016-07-13 [PMID: 27409807] (Blocking, Mouse)

Bioinformatics

• Gene Symbol: GDNF
• Uniprot:
  • P39905(Goat)