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Recombinant Human GDF-15 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Concentration
Lyoph

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Recombinant Human GDF-15 Protein Summary

Description
Recombinant bioactive protein for Human GDF-15

Source:E. coli
Amino Acid Sequence:(Q99988) - MARNGDDCPL GPGRCCRLHT VRASLEDLGW ADWVLSPREV QVTMCIGACP SQFRAANMHA QIKTSLHRLK PDTVPAPCCV PASYNPMVLI QKTDTGVSLQ TYDDLLAKDC HCI
Details of Functionality
The activity is determined by the inhibition of DU-145 cells and is typically 1-2 ug/ml.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
GDF15
Purity
>95%, by SDS-PAGE
Endotoxin Note
<0.01 ng/ug

Applications/Dilutions

Theoretical MW
26.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Sterile filtered and lyophilized with no additives
Concentration
Lyoph
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Centrifuge vial before opening. Reconstitute with sterile 5 mM acetic acid at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Alternate Names for Recombinant Human GDF-15 Protein

  • GDF15
  • GDF-15
  • growth differentiation factor 15
  • growth/differentiation factor 15
  • Macrophage inhibitory cytokine 1
  • MIC-1
  • MIC-1NSAID-activated gene 1 protein
  • MIC1Prostate differentiation factor
  • NAG-1
  • NAG-1NSAID-regulated gene 1 protein
  • NSAID (nonsteroidal inflammatory drug)-activated protein 1
  • PDF
  • PDFGDF-15
  • PLAB
  • PLABNRG-1
  • Placental bone morphogenetic protein
  • Placental TGF-beta
  • PTGF-beta
  • PTGFBPTGF-beta

Background

Growth and differentiation factor 15 (GDF-15) is a TGFBeta family member, made by in the placenta and heart tissues, that has a role in regulating inflammatory and apoptotic pathways. GDF-15 has become an immerging marker of early heart disease and has the potential as being used as a molecule for screening patients for early heart failure. Recombinant human GDF-15 (D-variant) is a non-glycosylated, disulfide linked homodimer. It is comprised of two identical 120 amino acid monomers and has a total molecular mass of 26.8 kDa. There is a His to an Asp substitution at position 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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FAQs for GDF-15 Recombinant Protein (NBP1-99414). (Showing 1 - 1 of 1 FAQ).

  1. Regarding the following product, could you let us know the Lyophilized buffer formulation and the Preservative/Concentration?
    • This protein (NBP1-99414) was lyophilized in sterile water. There is no preservative added. We recommend reconstituting with (200ul) sterilized 5 mM Acetic acid to a final concentration of 0.1mg/ml.

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Bioinformatics

Gene Symbol GDF15