Human GDF-15 ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
1.1 to 800 pg/mL |
| Sensitivity |
2 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 15% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
GDF15 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
GDF15 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GDF15. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Assay Diluent (Item E2): 5x concentrated buffer.
- Detection Antibody GDF-15 (Item F): Biotinylated anti-human GDF-15. Each vial isenough to assay half the microplate.
- GDF-15 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-humanGDF-15
- HRP-Streptavidin concentrates (Item G): 500x concentrated HRP-conjugatedStreptavidin.
- Standard Protein (Item C): Human GDF-15. I vial is enough to run each standard induplicate.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’- tetramethylbenzidine (TMB)in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20X concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human GDF-15 ELISA Kit (Colorimetric)
Background
Growth differentiation factor 15 (GDF15) is a transforming growth factor beta superfamily member that acts to regulate inflammatory and apoptotic responses in injured or diseased tissues. GDF15 is most strongly expressed in the liver, placenta and kidney.
GDF15 antibodies are useful for studies on inflamation and immune system research.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for GDF-15 ELISA Kit (KA1871). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.