GCR2 Antibody - BSA Free

Western Blot: GCR2 Antibody [NBP2-97163] - Lane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: 293T Whole Cell LysateLane D: U-251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:73 kDa

Immunoprecipitation: GCR2 Antibody [NBP2-97163] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-ECD rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ECD rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 73 kDaObserved band size :76 kDa

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

GCR2 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human GCR2 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ECD
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-5 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for GCR2 Antibody - BSA Free

• ecdysoneless homolog (Drosophila)
• GCR2
• HSGT1
• Protein ecdysoneless homolog
• protein SGT1
• SGT1
• Suppressor of GCR2
• suppressor of S. cerevisiae gcr2
• X

Background

Novel regulator of p53 stability and function. May also be a transcriptional activator required for the expression of glycolytic genes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ECD