GCLM Antibody (0U3J2)

Western Blot: GCLM Antibody (0U3J2) [NBP3-15380] - Western blot analysis of extracts of various cell lines, using GCLM antibody (NBP3-15380) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

• Reactivity: Hu
• Applications: WB
• Clone: 0U3J2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

GCLM Antibody (0U3J2) Summary

• Description: Novus Biologicals Rabbit GCLM Antibody (0U3J2) (NBP3-15380) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 175-274 of human GCLM (P48507). LYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GCLM
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for GCLM Antibody (0U3J2)

• Gamma-ECS regulatory subunit
• Gamma-glutamylcysteine synthetase regulatory subunit
• GCS light chain
• GLCLRglutamate--cysteine ligase regulatory subunit
• glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD)
• Glutamate--cysteine ligase modifier subunit
• glutamate-cysteine ligase regulatory protein
• glutamate-cysteine ligase, modifier subunit
• GSC light chain

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GCLM