GCLC Antibody

Western Blot: GCLC Antibody [NB600-544] - Western Blot analysis of GCLC from Human Cells.

Product Discontinued

GCLC Antibody Summary

• Immunogen: A synthetic peptide derived from corresponding to aa 295-313 of rat GCLC (heavy subunit of GCS)
• Localization: Cytoplasmic
• Specificity: Recognizes the heavy subunit of GCS at 73kDa
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GCLC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 10 ug/ml
  • Western Blot 2.5 - 5.0 ug/ml
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Monkey.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for GCLC Antibody

• EC 6.3.2.2
• Gamma-ECS
• Gamma-glutamylcysteine synthetase
• GCLC
• GCS heavy chain
• GCS
• GLCL
• GLCLC
• GLCLCGCL
• glutamate--cysteine ligase catalytic subunit
• glutamate-cysteine ligase, catalytic subunit

Background

Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlle by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GCLC Antibody (NB600-544)(1)

Publication using NB600-544

• Theodoratos A, Blackburn AC, Cappello J et al. Dichloroacetic acid up-regulates hepatic glutathione synthesis via the induction of glutamate-cysteine ligase. Biochemical Pharmacology. 2011-11-23 [PMID: 22127296]

Bioinformatics

• Gene Symbol: GCLC
• Entrez:
  • 2729(Human)
  • 14629(Mouse)
  • 25283(Rat)
• Uniprot:
  • P19468(Rat)
  • P48506(Rat)