GCLM Antibody (2B8) - Azide and BSA Free

Western Blot: GCLM Antibody (2B8) [H00002730-M01] - GCLM monoclonal antibody (M01), clone 2B8 Analysis of GCLM expression in HeLa.

Sandwich ELISA: GCLM Antibody (2B8) [H00002730-M01] - Detection limit for recombinant GST tagged GCLM is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 2B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GCLM Antibody (2B8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GCLM (AAH41809, 1 a.a. ~ 274 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSGASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS
• Specificity: GCLM (2B8)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GCLM
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 21540084). Mouse reactivity reported in scientific literature (PMID: 20146260).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GCLM Antibody (2B8) - Azide and BSA Free

• Gamma-ECS regulatory subunit
• Gamma-glutamylcysteine synthetase regulatory subunit
• GCS light chain
• GLCLRglutamate--cysteine ligase regulatory subunit
• glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD)
• Glutamate--cysteine ligase modifier subunit
• glutamate-cysteine ligase regulatory protein
• glutamate-cysteine ligase, modifier subunit
• GSC light chain

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GCLM Antibody (H00002730-M01)(7)

Publications using H00002730-M01

• Lu W, Chen Z, Zhang H et al. ZNF143 transcription factor mediates cell survival through upregulation of the GPX1 activity in the mitochondrial respiratory dysfunction Cell Death Dis 2012-11-15 [PMID: 23152058] (WB, Human)
• Abel EL, Bubel JD, Simper MS et al. Protection against 2-chloroethyl ethyl sulfide (CEES) - Induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway. Toxicol Appl Pharmacol. 2011-06-23 [PMID: 21723306]
• Zhang L, Gavin T, Barber DS, LoPachin RM. Role of the Nrf2-ARE pathway in acrylamide neurotoxicity. Toxicol Lett;205(1):1-7. 2011-08-10 [PMID: 21540084] (WB, Rat)
• Sharon N, Mor I, Golan-Lev T et al. Molecular and Functional Characterization of Gastrula Organizer Cells Derived from Human Embryonic Stem Cells. Stem Cells. 2011-02-15 [PMID: 21328508]
• Hardwick RN, Fisher CD, Canet MJ et al. Diversity in Antioxidant Response Enzymes in Progressive Stages of Human Nonalcoholic Fatty Liver Disease. Drug Metab Dispos. 2010-08-30 [PMID: 20805291]
• Yang H, Ko K, Xia M et al. Induction of avian musculoaponeurotic fibrosarcoma proteins by toxic bile acid inhibits expression of glutathione synthetic enzymes and contributes to cholestatic liver injury in mice. Hepatology 2010-04-01 [PMID: 20146260] (WB, Mouse, Human)
• Balamurugan AN, Akhov L, Selvaraj G, Pugazhenthi S. Induction of Antioxidant Enzymes by Curcumin and Its Analogues in Human Islets: Implications in Transplantation. Pancreas. 2009-05-01 [PMID: 19188863]

Bioinformatics

• Gene Symbol: GCLM
• Entrez:
  • 2730(Human)
• OMIM:
  • 601176(Human)
• Uniprot:
  • AAH41809(Human)