Gastrokine 1 Antibody [Unconjugated]

Western blot shows lysates of human stomach tissue. PVDF Membrane was probed with 1 µg/mL of Human Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6395) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Gastrokine 1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Gastrokine 1 was detected in immersion fixed paraffin-embedded sections of human stomach using Sheep Anti-Human Gastrokine 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6395) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to luminal part of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Gastrokine 1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Gastrokine 1
  Asn35-Asn199
  Accession # Q9NS71
• Specificity: Detects human Gastrokine 1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: GKN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Gastrokine 1 Antibody [Unconjugated]

• 18 kDa antrum mucosa protein
• AMP18
• AMP-18
• AMP18BRICHOS domain containing 1
• BRICD1
• CA11
• CA11MGC70354
• FOV
• Foveolin
• Gastrokine 1
• gastrokine-1
• GKN1
• Protein CA11

Background

Gastrokine 1 (GKN1; also CA11 and AMP-18) is an 18 kDa member of the CA11 protein family. It has limited expression, being restricted to mucous secreting pyloric atrial epithelium. Gastrokine 1 appears to promote epithelial proliferation and migration, and induce the formation of tight junctions between epithelial cells. Mature human Gastrokine 1 is 165 amino acids (aa) in length. Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 54-150) that contains a mitogenic sequence (aa 97-117). There is one potential alternative start site 14 aa upstream of the standard site that does not appear to be utilized. Over aa 21-185 (aa 35-199 of SwissProt Q9NS71), human Gastrokine 1 shares 65% aa identity with mouse Gastrokine 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Gastrokine 1 Antibody (AF6395)(1)

Publication using AF6395

• S Yao, HY Huang, X Han, Y Ye, Z Qin, G Zhao, F Li, G Hu, L Hu, H Ji Keratin 14-high subpopulation mediates lung cancer metastasis potentially through Gkn1 upregulation Oncogene, 2019-07-18;0(0):. 2019-07-18 [PMID: 31320708] (IHC, Human)

Bioinformatics

• Gene Symbol: GKN1
• Uniprot:
  • Q9NS71(Human)