Gastrokine 1 Antibody (2E5) - Azide and BSA Free

Western Blot: Gastrokine 1 Antibody (2E5) [H00056287-M01] - Analysis of GKN1 expression in transfected 293T cell line by GKN1 monoclonal antibody (M01), clone 2E5.Lane 1: GKN1 transfected lysate(20 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: Gastrokine 1 Antibody (2E5) [H00056287-M01] - Detection limit for recombinant GST tagged GKN1 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 2E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Gastrokine 1 Antibody (2E5) - Azide and BSA Free Summary

• Immunogen: GKN1 (AAH59778, 21 a.a. ~ 185 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NYNINVNDDNNNAGSGQQSVSVNNEHNVANVDNNNGWDSWNSIWDYGNGFAATRLFQKKTCIVHKMNKEVMPSIQSLDALVKEKKLQGKGPGGPPPKGLMYSVNPNKVDDLSKFGKNIANMCRGIPTYMAEEMQEASLFFYSGTCYTTSVLWIVDISFCGDTVEN
• Specificity: GKN1 - gastrokine 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GKN1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Gastrokine 1 Antibody (2E5) - Azide and BSA Free

• 18 kDa antrum mucosa protein
• AMP18
• AMP-18
• AMP18BRICHOS domain containing 1
• BRICD1
• CA11
• CA11MGC70354
• FOV
• Foveolin
• Gastrokine 1
• gastrokine-1
• GKN1
• Protein CA11

Background

The protein encoded by this gene is found to be down-regulated in human gastric cancer tissue as compared to normal gastric mucosa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Gastrokine 1 Antibody (H00056287-M01)(9)

Publications using H00056287-M01

• Alarcon-Millan J, Lorenzo-Nazario S, Jimenez-Wences H et al. Women with chronic follicular gastritis positive for Helicobacter pylori express lower levels of GKN1. Gastric Cancer. 2020-02-21 [PMID: 32086651]
• Stella C, Faraonio R, Federico A et al. GKN1 expression in gastric cancer cells is negatively regulated by miR-544a. Biochimie. 2019-09-08 [PMID: 31509760]
• Villano V, Di Stadio CS, Federico A et al. Gastrokine 1 mRNA in human sera is not informative biomarker for gastric cancer. J Negat Results Biomed 2016-07-25 [PMID: 27452910]
• Altieri F, Di Stadio CS, Severino V et al. Anti-amyloidogenic property of human gastrokine 1. Biochimie. 2014-08-17 [PMID: 25139219]
• Mao W, Chen J, Peng TL et al. Helicobacter pylori infection and administration of non-steroidal anti-inflammatory drugs down-regulate the expression of gastrokine-1 in gastric mucosa. Turk J Gastroenterol. 2012-06-01 [PMID: 22798109]
• Mao W, Chen J, Peng TL et al. Downregulation of gastrokine-1 in gastric cancer tissues and restoration of its expression induced gastric cancer cells to apoptosis. J Exp Clin Cancer Res. 2012-05-23 [PMID: 22621392]
• Kam SY, Hennessy T, Chua SC et al. Characterization of the Human Gastric Fluid Proteome Reveals Distinct pH-Dependent Protein Profiles: Implications for Biomarker Studies. J Proteome Res. 2011-08-15 [PMID: 21842849]
• Rippa E, La Monica G, Allocca R et Al. Overexpression of gastrokine 1 in gastric cancer cells induces Fas-mediated apoptosis J Cell Physiol 2011-10-13 [PMID: 21792914]
• Rippa E, La Monica G, Allocca R et al. Overexpression of gastrokine 1 in gastric cancer cells induces fas-mediated apoptosis. J Cell Physiol. 2010-12-28 [PMID: 21190249]

Bioinformatics

• Gene Symbol: GKN1
• Entrez:
  • 56287(Human)
• OMIM:
  • 606402(Human)
• Uniprot:
  • AAH59778(Human)