Gas6 Antibody (126618)

• Reactivity: Mu
• Applications: WB
• Clone: 126618
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Gas6 Antibody (126618) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Gas6
  Asp115-Pro674 (Del Pro530)
  Accession # Q61592.2
• Specificity: Detects mouse Gas6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human Gas6.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: GAS6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Gas6 Antibody (126618)

• AXLLG
• AXLLGAXL stimulatory factor
• AXSFAXL receptor tyrosine kinase ligand
• DKFZp666G247
• FLJ34709
• Gas6
• GAS-6
• growth arrest-specific 6
• growth arrest-specific protein 6

Background

Growth arrest-specific gene 6 (Gas6) was initially characterized as a gene whose expression was up-regulated in serum starved NIH 3T3 fibroblasts and whose expression is down-regulated during growth induction. Mouse Gas6 is a 673 amino acid protein that shares 81% identity to its human homolog. Gas6 is a member of the vitamin K-dependent family of proteins that includes human protein S, a negative coregulator in the blood coagulation pathway. Gas6 and protein S share structural motifs that characterize this family: an extensively gamma -carboxylated amino terminus (Gla domain), four EGF-like repeats, and a carboxy terminus containing globular (G) domains with homology to steroid hormone-binding globulin. It is a ligand for the Axl (Ufo/Ark), Sky (Dtk/Tyro3/Rse/Brt/Tif), and Mer (Eyk) families of tyrosine kinase receptors. Gas6 binds to these receptors via tandem G domains at its C-terminus. Gas6 is ubiquitously expressed, but most abundantly in lung, intestine, bone marrow and endothelium. Gas6 has been implicated in a variety of biological processes. It serves as a mitogen for fibroblasts, endothelial cells, neural cells, vascular smooth muscle cells and several tumor-derived cell lines. In addition, Gas6 prevents apoptosis, independent of its mitogenic activity. Gas6 is capable of inducing cell adhesion and chemotaxis in specific cell types. It also supports vitamin K-independent hematopoiesis when expressed by stromal cells. Gas6 signal transduction following Axl/Sky/Mer receptor activation has been reported to occur through such diverse signaling pathways as PI3K, MAP kinase, Src, Ras and beta -catenin.

1. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
2. Goruppi, S. et al. (1996) Oncogene 12:471.
3. Crosier, K and P. Crosier (1997) Pathology 29:131.
4. Dormady, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12260.
5. Goruppi, S. et al. (2001) Mol. Cell Biol. 21:902.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Gas6 Antibody (MAB986)(4)

Publications using MAB986

• K Zubeidat, Y Jaber, Y Saba, O Barel, R Naamneh, Y Netanely, Y Horev, L Eli-Bercho, A Shhadeh, O Yosef, E Arbib, G Betser-Coh, C Nadler, H Shapiro, E Elinav, DJ Aframian, A Wilensky, AH Hovav Microbiota-dependent and -independent postnatal development of salivary immunity Cell Reports, 2023-01-07;42(1):111981. 2023-01-07 [PMID: 36640306] (IHC, Mouse)
• ME Fane, Y Chhabra, GM Alicea, DA Maranto, SM Douglass, MR Webster, VW Rebecca, GE Marino, F Almeida, BL Ecker, DJ Zabransky, L Hüser, T Beer, HY Tang, A Kossenkov, M Herlyn, DW Speicher, W Xu, X Xu, EM Jaffee, JA Aguirre-Gh, AT Weeraratna Stromal changes in the aged lung induce an emergence from melanoma dormancy Nature, 2022-06-01;606(7913):396-405. 2022-06-01 [PMID: 35650435] (Western Blot, Mouse)
• Gautier Tejedor, Patricia Luz-Crawford, Audrey Barthelaix, Karine Toupet, Sébastien Roudières, François Autelitano et al. MANF Produced by MRL Mouse-Derived Mesenchymal Stem Cells Is Pro-regenerative and Protects From Osteoarthritis Frontiers in Cell and Developmental Biology 2021-03-02 [PMID: 33738280]
• Anwar A, Keating AK, Joung D, Sather S, Kim GK, Sawczyn KK, Brandao L, Henson PM, Graham DK Mer tyrosine kinase (MerTK) promotes macrophage survival following exposure to oxidative stress. J. Leukoc. Biol., 2009-04-22;86(1):73-9. 2009-04-22 [PMID: 19386698] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: GAS6
• Uniprot:
  • Q61592.2()