GAPDH-2 Overexpression Lysate (Native)

Western Blot: GAPDHS Overexpression Lysate (Adult Normal) [NBL1-10968] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GAPDHS.

• Reactivity: Hu
• Applications: WB

GAPDH-2 Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC205362 and based on accession number NM_014364. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens glyceraldehyde-3-phosphate dehydrogenase, spermatogenic (GAPDHS), mRNA.
• Gene: GAPDHS

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 44.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for GAPDH-2

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GAPDH-2 Overexpression Lysate (Native)

• EC 1.2.1
• EC 1.2.1.12
• GAPD2
• GAPD2HSD-35
• GAPDH2
• GAPDH-2
• GAPDH-2glyceraldehyde-3-phosphate dehydrogenase, testis-specific
• GAPDHS
• GAPDS
• glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• HSD-35
• Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2
• Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

Background

This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: GAPDHS
• Entrez:
  • 26330(Human)
  • 14447(Mouse)
  • 66020(Rat)