GAP-43 Antibody (1E3) [Allophycocyanin]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 1E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

GAP-43 Antibody (1E3) [Allophycocyanin] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: C-terminal peptide of rat and mouse GAP43 (KEDPEADQEHA) to which an N-terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.
• Localization: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection > growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction > synapse. Cell projection > filopodium membrane; Peripheral membrane protein. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.
• Marker: Neuronal Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GAP43
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: This GAP43 (1E3) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 43 kDa.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for GAP-43 Antibody (1E3) [Allophycocyanin]

• Axonal membrane protein GAP-43
• B-50
• Basp2
• calmodulin-binding protein P-57
• GAP43
• GAP-43
• growth associated protein 43
• Growth-associated protein 43
• nerve growth-related peptide GAP43
• Neural phosphoprotein B-50
• Neuromodulin
• neuron growth-associated protein 43
• PP46
• protein F1

Background

GAP43 (Growth associated protein 43) is a crucial component for regenerative response in the nervous system. GAP-43 is normally produced by neurons during developmental growth and axonal regeneration, but it is also expressed in specific regions of the normal adult nervous system. The protein is present at high levels in neuronal growth cones during development and axonal regeneration.

Heterozygous GAP3 knockout mice with GAP-43 levels reduced by one-half display significant memory impairments in cued conditioning, or on tests of nociceptive or auditory perception. Differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. Therefore, the neuronal precursor pool in the cerebellum fails to grow resulting in a cerebellum that is significantly smaller than normal. Total GAP-43 deletion is lethal within days of birth, demonstrating that GAP-43 is critical for the proper development of the mammalian CNS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GAP43