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Rat Galectin-7 ELISA Kit (Chemiluminescence)

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Rat Galectin-7 ELISA Kit (Chemiluminescence) - Standard Curve Reference
Rat Galectin-7 ELISA Kit (Chemiluminescence) - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL

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Rat Galectin-7 ELISA Kit (Chemiluminescence) Summary

Specificity
This kit recognizes Rat GAL7 in samples. No significant cross-reactivity or interference between Rat GAL7 and analogues was observed.
Standard Curve Range
31.25-2000 pg/mL
Sensitivity
18.75 pg/mL
Assay Type
Sandwich-CLIA
Inter-Assay
CV% < 10.92%
Intra-Assay
CV% < 8.65%
Spike Recovery
87-113%
Sample Volume
100 uL
Kit Type
ELISA Kit (Chemiluminescence)
Gene
LGALS7

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. HRP Conjugate Diluent
  7. Micro CLIA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Substrate Reagent A
  13. Substrate Reagent B

Alternate Names for Rat Galectin-7 ELISA Kit (Chemiluminescence)

  • GAL7
  • Gal-7
  • galectin 7
  • Galectin7
  • Galectin-7
  • HKL-14
  • lectin, galactoside-binding, soluble, 7
  • LGALS7
  • LGALS7A
  • p53-induced gene 1 protein
  • PI7
  • PIG1
  • TP53I1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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AF1152
Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog&amp;nbsp;# FAB3832P) and either (A) Normal Goat IgG Control (Catalog&amp;nbsp;# AB-108-C) or (B) Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Galectin‑1 at approximately 17&amp;nbsp;kDa (as indicated) using 1&amp;nbsp;µg/mL of Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) followed by 1:50&amp;nbsp;dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing&amp;nbsp;conditions and using the12-230 kDa separation system.
Galectin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 1.7&amp;nbsp;µg/mL Goat Anti-Human Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1152) overnight at 4&amp;nbsp;°C. Tissue was stained with the Anti-Goat HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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(12 Publications)
Species: Hu
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
Galanin Antibody
NBP2-33582
Immunocytochemistry/Immunofluorescence: Galanin Antibody [NBP2-33582] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Galanin Antibody [NBP2-33582] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Galanin Antibody [NBP2-33582] - Staining of human pituitary gland shows moderate to strong cytoplasmic positivity in endocrine glandular cells.
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(1 Publication)
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
Galectin-3
Antibody [Unconjugate
AF1197
Western blot shows lysates of MCF‑7 human breast cancer cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, C6 rat glioma cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.2&amp;nbsp;µg/mL of Goat Anti-Mouse Galectin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF017). A specific band was detected for Galectin‑3 at approximately 28&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Galectin‑3 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c., cross-section through the eye) using Goat Anti-Human Galectin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197 ) at 15&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the Anti-Goat HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western lane view shows lysates of HT‑2 mouse T cell line and Balb/3T3 mouse embryonic fibroblast cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Galectin‑3 at approximately 44&amp;nbsp;kDa (as indicated) using 10&amp;nbsp;µg/mL of Goat Anti-Mouse Galectin‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing&amp;nbsp;conditions and using the 12-230 kDa separation system.&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;
(2 Reviews)
(15 Publications)
Species: Hu, Mu, Rt
Applications: IHC, Simple Western, WB
Galectin-10
Antibody [Unconjugate
AF5447
Western blot shows lysates of HL‑60 human acute promyelocytic leukemia cell line. PVDF membrane was probed with 1&amp;nbsp;µg/mL of Goat Anti-Human Galectin‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5447) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF019). A specific band was detected for Galectin‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Human PBMC lymphocytes were stained with Goat&amp;nbsp;Anti-Human&amp;nbsp;Galectin‑10 Antigen Affinity‑purified Polyclonal Antibody (Catalog&amp;nbsp;# AF5447) followed by CFS-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# F0109) and Mouse Anti-Human CD25/IL-2 R alpha Allophycocyanin-conjugated Monoclonal Antibody (Catalog&amp;nbsp;# FAB1020A).Quadrant markers were set based on control antibody staining (Catalog&amp;nbsp;# AB‑108‑C).
(1 Publication)
Species: Hu
Applications: CyTOF-ready, ICFlow, WB
Galectin-4
Antibody (9Z2B5)
NBP3-16249
Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunohistochemistry of paraffin-embedded human appendix using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunohistochemistry of paraffin-embedded mouse colon using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry: Galectin-4 Antibody (9Z2B5) [NBP3-16249] - Immunofluorescence analysis of rat rectum using Galectin-4 Rabbit mAb (NBP3-16249) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
GCET2 Antibody
NBP1-82693
Western Blot: GCET2 Antibody [NBP1-82693] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: GCET2 Antibody [NBP1-82693] - Staining of human tonsil shows cytoplasmic positivity in reaction center cells.
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GALK1 Antibody
NBP1-81853
Western Blot: GALK1 Antibody [NBP1-81853] - Analysis using Anti-GALK1 antibody NBP1-81853 (A) shows similar pattern to independent antibody NBP1-81854 (B).
Immunocytochemistry/Immunofluorescence: GALK1 Antibody [NBP1-81853] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Western Blot: GALK1 Antibody [NBP1-81853] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
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240) - BSA Free
NB200-103
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
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MMP-9 [HRP]
DMP900
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AF6667
Western blot shows lysates of mouse stomach tissue. PVDF Membrane was probed with 0.5&amp;nbsp;µg/mL of Mouse Galectin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6667) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog&amp;nbsp;# HAF016). A specific band was detected for Galectin‑2 at approximately 15&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Galectin‑2 was detected in perfusion fixed frozen sections of mouse intestine using Mouse Galectin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6667) at 5&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Tissue was stained using the Anti-Sheep HRP-DAB Cell &amp;amp; Tissue Staining Kit (brown; Catalog&amp;nbsp;# CTS019) and counterstained with hemotoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
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beta-Galactosidase-1/G ...
NBP2-45731
Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
(6 Publications)
Species: Ca, Hu, Pm
Applications: IHC, IHC-P, WB
GALT Antibody
(4C11)
H00002592-M01
Western Blot: GALT Antibody (4C11) [H00002592-M01] - Analysis of GALT expression in transfected 293T cell line by GALT monoclonal antibody (M01), clone 4C11.Lane 1: GALT transfected lysate(43 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: GALT Antibody (4C11) [H00002592-M01] - Analysis of monoclonal antibody to GALT on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1 ug/ml.
Sandwich ELISA: GALT Antibody (4C11) [H00002592-M01] - Detection limit for recombinant GST tagged GALT is approximately 0.3ng/ml as a capture antibody.
(1 Review)
(1 Publication)
Species: Hu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
Caspase-3 Antibody
(31A1067) - (Pro and ...
NB100-56708
Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on HeLa cells with Caspase-3 Antibody (31A1067) - (Pro and Active) NB100-56708 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&amp;D Systems).
Flow Cytometry: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - An intracellular stain was performed on NIH3T3 cells with Caspase-3 Antibody (31A1067) - (Pro and Active) Antibody NB100-56708AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Paclitaxel in combination with MWE retarded tumor growth in a human bladder carcinoma TSGH 8301 xenograft model. The levels of total (t-PTEN) and phospho-PTEN (p-PTEN) and Caspase 3 in the tumor specimens were determined by Western blotting and then quantified using beta-actin as the protein loading control; the results are expressed as a percentage of the control. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep20417), licensed under a CC-BY licence.
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Applications: CyTOF-ready, EM, Flow, HEStain, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, KD, KO, Simple Western, WB
Galectin-8
Antibody [Unconjugate
AF1305
Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1&amp;nbsp;µg/mL of Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF019). A specific band was detected for Galectin‑8 at approximately 35&amp;nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Galectin‑8 at approximately 40&amp;nbsp;kDa (as indicated) using 10&amp;nbsp;µg/mL of Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing&amp;nbsp;conditions and using the12-230 kDa separation system.
Galectin‑8 was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human Galectin‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1305) at 15&amp;nbsp;µg/mL overnight at 4&amp;nbsp;°C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell &amp;amp; Tissue Staining Kit&amp;nbsp; (brown; Catalog&amp;nbsp;# CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(6 Reviews)
(14 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
Cytokeratin 14
Antibody (LL002)
NBP2-34270
Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).
Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).
(2 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western
14-3-3
sigma/Stratifin Antib
NBP2-27072
Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - analysis of 14-3-3 sigma (248 amino acid isoform) using 14-3-3 sigma polyclonal antibody. Human A431 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) HeLa cell lysate probed with 14-3-3 sigma antibody at 3 ug/ml.
Western Blot: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Analysis of 14-3-3 sigma (169 amino acid isoform) using 14-3-3 sigma antibody. Human brain tissue in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain tissue probed with 14-3-3 sigma antibody at 3 ug/ml.
Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody [NBP2-27072] - Formalin-fixed, paraffin-embedded human tonsil (squamous epithelium) probed with 14-3-3 sigma antibody at 10 ug/ml.
Species: Am, Bv, Ca, Eq, Hu, Ma-Op, Pm
Applications: IHC, IHC-P, KD, WB
Rat Galectin-7
ELISA Kit (Chemilumin ...
NBP2-67976
Rat Galectin-7 ELISA Kit (Chemiluminescence)
Species: Rt
Applications: ELISA

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Western blot shows lysates of human skin tissue. PVDF membrane was probed with 1&nbsp;µg/mL of Goat Anti-Human Galectin‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&nbsp;# HAF017). A specific band was detected for Galectin‑7 at approximately 15&nbsp;kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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  Rat Galectin-7 ELISA Kit (Chemiluminescence) Manual
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Bioinformatics

Gene Symbol LGALS7