Galectin-3 Antibody - BSA Free

Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795] - Staining in human colon and liver tissues using NBP1-89795 antibody. Corresponding LGALS3 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795] - Staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795] - Staining of human fallopian tube shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP1-89795] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Analysis in human cell lines SK-MEL-30 and U-251MG using Anti-LGALS3 antibody. Corresponding LGALS3 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Galectin-3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Galectin-3 Antibody - BSA Free (NBP1-89795) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SSGQPSATGAYPATGPYGAPAGPLIVPYNLPLPGGVVPRMLITILGTVKPNANRIALDFQRGNDVAFHFNPRFNENNRRVIVCNTKLDNNWGREERQSVFPFESGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRVK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LGALS3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Galectin-3 Protein (NBP1-89795PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (83%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Galectin-3 Antibody - BSA Free

• AGE-R3
• Carbohydrate-binding protein 35
• CBP 35,35 kDa lectin
• CBP35
• GAL3
• Gal-3
• galactin-3
• Galactose-specific lectin 3
• Galactoside-binding protein
• GALBPCBP35
• Galectin3
• Galectin-3
• GALIG
• IgE-binding protein
• L29
• L31
• L-31
• Laminin-binding protein
• Lectin L-29
• lectin, galactoside-binding, soluble, 3
• LGALS2
• LGALS3
• Mac-2 antigen
• Mac-2
• MAC2GAL3

Background

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LGALS3