Galectin-1 Antibody (RM0081-9J26) - Azide and BSA Free

Western Blot: Galectin-1 Antibody (RM0081-9J26) [NBP1-21721] - Analysis of mouse placental tissue lysates.

• Reactivity: Mu
• Applications: WB, IHC
• Clone: RM0081-9J26
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Galectin-1 Antibody (RM0081-9J26) - Azide and BSA Free Summary

• Description: Novus Biologicals Rat Galectin-1 Antibody (RM0081-9J26) - Azide and BSA Free (NBP1-21721) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Mouse recombinant Galectin-1
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: LGALS1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for Galectin-1 Antibody (RM0081-9J26) - Azide and BSA Free

• 14 kDa laminin-binding protein
• 14 kDa lectin
• Beta-galactoside-binding lectin L-14-I
• BHL
• DKFZp686E23103
• GAL1
• gal-1
• Galaptin
• galectin 1
• Galectin1
• Galectin-1
• GBP
• HBL
• HLBP14
• HPL
• L-14
• Lactose-binding lectin 1
• Lectin galactoside-binding soluble 1
• lectin, galactoside-binding, soluble, 1
• LGALS1
• Putative MAPK-activating protein PM12
• S-Lac lectin 1

Background

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 1 may act as an autocrine negative growth factor that regulates cell proliferation. It also may regulate cell apoptosis and cell differentiation. It binds beta-galactoside, CD45, CD3 and CD4. Galectin 1 inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. It is found in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney, placenta and hematopoietic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Galectin-1 Antibody (NBP1-21721). (Showing 1 - 1 of 1 FAQ).

1. Would you please tell me how mouse heart sample was prepared for the WB image from NBP1-95445?
   • The protocol for tissue samples preparation for the WB with Galectin 1 antibody (# NBP1-95445 ) is as follows: 1. Wash the tissue with cold PBS. 2. Chop the tissue into small pieces, tip tissue sample into liquid nitrogen. 3. Start crushing tissue sample with pestle. Crushing sample to a powder before the liquid nitrogen completely evaporates. 4. Add appropriate volume of 1X hot lysis buffer, 100C, 5min. 5. Sonicate the tissue for 10-15seconds. 6. Spin tissue debris down at 10000rpm, 10min. 7. Carefully remove the lipid on the surface of the supernatant. Save supernatant as whole tissue lysate, and discard the pellet. 8. Determine the protein concentration by BCA protein assay. 9. Adjust concentration to 2 mg/ml with Lysis buffer. 10. Add an equal volume of 2X Laemmli Sample Buffer (final concentration of 1 mg/ml). 11. Boil each cell lysate in sample buffer at 100 C for 5 min and aliquot.

Bioinformatics

• Gene Symbol: LGALS1
• Entrez:
  • 3956(Human)