Galectin-1 Antibody (933133) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Galectin-1 Monoclonal Antibody (Catalog # MAB1152) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin-1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Galectin-1 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Galectin-1 Monoclonal Antibody (Catalog # MAB1152) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysate of A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑1 at approximately 16 kDa (as indicated) using 100 µg/mL of Mouse Anti-Human Galectin‑1 Monoclonal Antibody (Catalog # MAB1152) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western blot shows lysates of HAP1 parental cell line and Galectin‑1 knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with Mouse Anti-Human Galectin‑1 Monoclonal Antibody (Catalog # MAB1152) followed by HRP-conjugated secondary antibody. A specific band was detected for Galectin‑1 at approximately 14.7 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. Primary antibody dilution used: 2 µg/mL. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, KO
• Clone: 933133
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Galectin-1 Antibody (933133) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Galectin-1
  Met1-Asp135
  Accession # P09382
• Specificity: Detects human Galectin-1 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LGALS1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Knockout Validated
  • Simple Western 100 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-1 Antibody (933133) [Unconjugated]

• 14 kDa laminin-binding protein
• 14 kDa lectin
• Beta-galactoside-binding lectin L-14-I
• BHL
• DKFZp686E23103
• GAL1
• gal-1
• Galaptin
• galectin 1
• Galectin1
• Galectin-1
• GBP
• HBL
• HLBP14
• HPL
• L-14
• Lactose-binding lectin 1
• Lectin galactoside-binding soluble 1
• lectin, galactoside-binding, soluble, 1
• LGALS1
• Putative MAPK-activating protein PM12
• S-Lac lectin 1

Background

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins. Galectin-1, also known as L-14, BHL and galaptin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, liver, prostate, lymph nodes, spleen, thymus, placenta, testis, retina, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth and proliferation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Human and mouse galectin-1 share about 88% amino acid sequence similarity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Galectin-1 Antibody (MAB1152)(5)

Publications using MAB1152

• Nagata, M;Ikuse, T;Tokushima, K;Arai, N;Jimbo, K;Kudo, T;Shimizu, T; High galectin expression in Helicobacter pylori-infected gastric mucosa in childhood Pediatrics and neonatology 2024-08-27 [PMID: 39244403] (Immunohistochemistry, Human)
• E Tarricone, E Mattiuzzo, E Belluzzi, R Elia, A Benetti, R Venerando, V Vindigni, P Ruggieri, P Brun Anti-Inflammatory Performance of Lactose-Modified Chitosan and Hyaluronic Acid Mixtures in an In Vitro Macrophage-Mediated Inflammation Osteoarthritis Model Cells, 2020-05-26;9(6):. 2020-05-26 [PMID: 32466461] (Western Blot, Human)
• Sarka Tumova, Michael J. Houghton, Gary Williamson The effect of quercetin on endothelial cells is modified by heterocellular interactions Food & Function 5/1/2020 [PMID: 32363357]
• Sarka Tumova, Michael J. Houghton, Gary Williamson The effect of quercetin on endothelial cells is modified by heterocellular interactions Food & Function 2020-05-01 [PMID: 32363357] (Simple Western, Human)
• Chao Gao, Melinda S. Hanes, Lauren A. Byrd-Leotis, Mohui Wei, Nan Jia, Robert J. Kardish et al. Unique Binding Specificities of Proteins Towards Isomeric Asparagine-linked Glycans Cell Chemical Biology 4/1/2019 [PMID: 30745240]

Bioinformatics

• Gene Symbol: LGALS1
• Uniprot:
  • P09382()