GADD45G Antibody (OTI2F12) - Azide and BSA Free

Western Blot: GADD45G Antibody (OTI2F12) - Azide and BSA Free [NBP2-71646] - GADD45G protein level by using anti-GADD45G antibody in human CRC cells.

Immunocytochemistry/Immunofluorescence: GADD45G Antibody (OTI2F12) - Azide and BSA Free [NBP2-71646] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY GADD45G.

Immunohistochemistry: GADD45G Antibody (OTI2F12) - Azide and BSA Free [NBP2-71646] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: GADD45G Antibody (OTI2F12) - Azide and BSA Free [NBP2-71646] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G.

Immunohistochemistry: GADD45G Antibody (OTI2F12) - Azide and BSA Free [NBP2-71646] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: OTI2F12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GADD45G Antibody (OTI2F12) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human GADD45G(NP_006696) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GADD45G
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:4000
• Theoretical MW: 16.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for GADD45G Antibody (OTI2F12) - Azide and BSA Free

• CR6DNA damage-inducible transcript 2 protein
• Cytokine-responsive protein CR6
• DDIT-2
• DDIT2GADD45-gamma
• growth arrest and DNA damage-inducible protein GADD45 gamma
• growth arrest and DNA-damage-inducible, gamma
• GRP1717 kD

Background

GADD45G is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GADD45G