GADD45G Antibody (OTI2F12)

Images

 
Western Blot: GADD45G Antibody (OTI2F12) [NBP2-45807] - GADD45G protein level by using anti-GADD45G antibody in human CRC cells.
Immunocytochemistry/ Immunofluorescence: GADD45G Antibody (OTI2F12) [NBP2-45807] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY GADD45G.
Immunohistochemistry: GADD45G Antibody (OTI2F12) [NBP2-45807] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: GADD45G Antibody (OTI2F12) [NBP2-45807] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD45G.
Immunohistochemistry: GADD45G Antibody (OTI2F12) [NBP2-45807] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
OTI2F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GADD45G Antibody (OTI2F12) Summary

Immunogen
Full length human recombinant protein of human GADD45G(NP_006696) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GADD45G
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:4000
Theoretical MW
16.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-45807.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GADD45G Antibody (OTI2F12)

  • CR6DNA damage-inducible transcript 2 protein
  • Cytokine-responsive protein CR6
  • DDIT-2
  • DDIT2GADD45-gamma
  • growth arrest and DNA damage-inducible protein GADD45 gamma
  • growth arrest and DNA-damage-inducible, gamma
  • GRP1717 kD

Background

GADD45G is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45G is highly expressed in placenta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GADD45G Antibody (NBP2-45807)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GADD45G Antibody (NBP2-45807) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GADD45G