Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Sample (50 ug of whole cell lysate) A: rat brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] - Paraformaldehyde-fixed HeLa, using GAD65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2500.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7.0).
Immunogen affinity purified
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 2 Reviews rated 5 using NBP1-33284 in the following application:
glutamate decarboxylase 2 (pancreatic islets and brain, 65kD)
glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
glutamate decarboxylase 2
Glutamate decarboxylase 65 kDa isoform
Glutamate decarboxylase-2 (pancreas)
MGC161607,65 kDa glutamic acid decarboxylase
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.