GAD2/GAD65 Antibody

Images

 
Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Sample (50 ug of whole cell lysate) A: rat brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] - Paraformaldehyde-fixed HeLa, using GAD65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2500.
Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-33284] - Paraffin-embedded RT2 xenograft; antibody dilution 1:500.
Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Sample (50 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE; antibody diluted at 1:5000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

GAD2/GAD65 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary.
Localization
Cytoplasm, cytosol, Cytoplasmic vesicle, Cell junction, synapse, presynaptic cell membrane, Golgi apparatus membrane
Marker
GABAergic Neuronal Marker
Predicted Species
Porcine (95%), Bovine (93%), Canine (96%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GAD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-33284 in the following applications:

Publications
Read Publications using
NBP1-33284 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Zebrafish (86%), Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GAD2/GAD65 Antibody

  • EC 4.1.1
  • EC 4.1.1.15
  • GAD2
  • GAD65
  • GAD-65
  • GAD65MGC161605
  • glutamate decarboxylase 2 (pancreatic islets and brain, 65kD)
  • glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
  • glutamate decarboxylase 2
  • Glutamate decarboxylase 65 kDa isoform
  • Glutamate decarboxylase-2 (pancreas)
  • MGC161607,65 kDa glutamic acid decarboxylase

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GAD2/GAD65 Antibody (NBP1-33284)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(1)
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Rat
(2)
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Reviews for GAD2/GAD65 Antibody (NBP1-33284) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2 application: WB, IF.

Reviews using NBP1-33284:
Filter by Applications
WB
(1)
IF
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
YONGJIE LAI
WB Mouse 10/01/2014
View

Summary

ApplicationWestern Blot
Sample Testedmouse brain lysate
SpeciesMouse
 
reviewed by:
YONGJIE LAI
IF Human 10/01/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedpCMV-GAD65 plasmid transfected HEK293T cells
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GAD2/GAD65 Antibody (NBP1-33284) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for GAD2/GAD65 Antibody (NBP1-33284)

Discover related pathways, diseases and genes to GAD2/GAD65 Antibody (NBP1-33284). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

 
YONGJIE LAI
10/01/2014
Application: WB
Species: Mouse

 
YONGJIE LAI
10/01/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol GAD2
Entrez
Uniprot