Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] - DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD65 stained by GAD65 antibody ...read more
Immunocytochemistry/ Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] - Paraformaldehyde-fixed HeLa, using GAD65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2500.
For IHC-P, HIER with EDTA buffer pH 8.0 is recommended.
65 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 2 Reviews rated 5 using NBP1-33284 in the following applications:
Expected cross reactivity based on sequence homology: Zebrafish (86%).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Immunogen affinity purified
Alternate Names for GAD2/GAD65 Antibody
glutamate decarboxylase 2 (pancreatic islets and brain, 65kD)
glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
glutamate decarboxylase 2
Glutamate decarboxylase 65 kDa isoform
Glutamate decarboxylase-2 (pancreas)
MGC161607,65 kDa glutamic acid decarboxylase
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for GAD2/GAD65 Antibody (NBP1-33284)(2)
We have publications tested in 1 confirmed species: Rat.
We have publications tested in 2 applications: ICC/IF, WB.
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