Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Sample (50 ug of whole cell lysate) A: rat brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] - Paraformaldehyde-fixed HeLa, using GAD65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2500.
For IHC-P, HIER with EDTA buffer pH 8.0 is recommended.
65 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 2 Reviews rated 5 using NBP1-33284 in the following applications:
Expected cross reactivity based on sequence homology: Zebrafish (86%), Rhesus Monkey (100%).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Immunogen affinity purified
Alternate Names for GAD2/GAD65 Antibody
glutamate decarboxylase 2 (pancreatic islets and brain, 65kD)
glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
glutamate decarboxylase 2
Glutamate decarboxylase 65 kDa isoform
Glutamate decarboxylase-2 (pancreas)
MGC161607,65 kDa glutamic acid decarboxylase
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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