GABPA Antibody (8C1B10)

Western Blot: GABPA Antibody (8C1B10) [NBP1-47416] - Analysis using GABPA mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY GABPA cDNA (2).

Immunocytochemistry/Immunofluorescence: GABPA Antibody (8C1B10) [NBP1-47416] - Analysis of Hela cells using GABPA mouse mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Western Blot: GABPA Antibody (8C1B10) [NBP1-47416] - Analysis using GABPA mouse mAb against Hela (1), A549 (2), MCF-7 (3), NIH/3T3 (4) and SMMC-7721 (5) cell lysate.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 8C1B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GABPA Antibody (8C1B10) Summary

• Description: Novus Biologicals Mouse GABPA Antibody (8C1B10) (NBP1-47416) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human GABPA (aa120-190) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GABPA
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for GABPA Antibody (8C1B10)

• E4TF1-60
• E4TF1ATranscription factor E4TF1-60
• GA binding protein transcription factor, alpha subunit 60kDa
• GA-binding protein alpha chain
• GA-binding protein transcription factor, alpha subunit (60kD)
• GABP subunit alpha
• human nuclear respiratory factor-2 subunit alpha, 10
• NFT2
• NRF2
• NRF2A
• nuclear respiratory factor 2 alpha subunit
• Nuclear respiratory factor 2 subunit alpha

Background

This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GABPA
• Entrez:
  • 2551(Human)