GABA-AR alpha 6 Antibody

Product Discontinued

GABA-AR alpha 6 Antibody Summary

• Immunogen: Peptide with sequence C-PATGGTSKIDQYSR corresponding to internal region (near C-Terminus) according to NP_032094.2.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001093111.1; NP_032094.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GABRA6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments gave an approx. 75 kDa band in mouse fetal brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 49.8 kDa band according to NP_032094.2. The 75 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered to be "Innovators Grade". Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. These antibodies are directed against antigens that have limited or no commercial antibodies available for them, or for which there are limited expression data available to us. In an effort to encourage customer feedback, Novus has made these antibodies available through our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details.

Alternate Names for GABA-AR alpha 6 Antibody

• GABA A R alpha 6
• GABA subunit A receptor alpha 6
• GABA(A) receptor subunit alpha-6
• GABAAR alpha 6
• GABAARa6
• GABRA6
• gamma-aminobutyric acid (GABA) A receptor, alpha 6
• gamma-aminobutyric acid receptor subunit alpha-6
• MGC116903
• MGC116904

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics. The alpha 6 subunit is expressed in cerebellar granule cells. Though it is concentrated at synapses, it is found on extrasynaptic membranes, as well.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GABA-AR alpha 6 Antibody (NBP2-26070)(1)

Publication using NBP2-26070

• Green EK, Grozeva D, Moskvina V et al. Variation at the GABAA receptor gene, Rho 1 (GABRR1) associated with susceptibility to bipolar schizoaffective disorder. Am J Med Genet B Neuropsychiatr Genet 2010-10-05 [PMID: 20583128]

Bioinformatics

• Gene Symbol: GABRA6
• Entrez:
  • 2559(Human)
  • 14399(Mouse)
  • 29708(Rat)