GABA-AR alpha 3 Antibody

Images

 
Genetic Strategies: Western Blot: GABA A R alpha 3 Antibody [NB100-61096] - Rat brain lysates from wild type (Control) and a3-knockout (a3-K/O) animals showing specific immunolabeling of the ~51k a3-subunit of ...read more
Immunohistochemistry: GABA A R alpha 3 Antibody [NB100-61096] - Immunostaining of rat amygdala showing labelling of GABA A R alpha 3

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

GABA-AR alpha 3 Antibody Summary

Immunogen
Synthetic peptide from the N-terminal region of the alpha 3 subunit
Specificity
Specific for the ~51k alpha 3-subunit of the GABAA receptor in Western blots. Labeling is absent in alpha 3-subunit knockout animals.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GABRA3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 19959723)
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-61096 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for GABA-AR alpha 3 Antibody

  • GABA A R alpha 3
  • GABA(A) receptor subunit alpha-3
  • GABAARa3
  • GABRA3
  • gamma-aminobutyric acid (GABA) A receptor, alpha 3
  • gamma-aminobutyric acid receptor subunit alpha-3
  • gamma-animobutyric acid (GABA) A receptor, alpha 3
  • MGC33793

Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Poltl et al., 2003).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GABA-AR alpha 3 Antibody (NB100-61096)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GABRA3
Entrez
Uniprot