GABA-A R beta 3 Antibody (S87-25)

Images

 
Western Blot: GABA-A R beta 3 Antibody (S87-25) [NBP1-47613] - analysis of Rat brain membrane lysate showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S87-25. Load: ...read more
Immunohistochemistry-Frozen: GABA-A R beta 3 Antibody (S87-25) [NBP1-47613] - Tissue: Brain. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
S87-25
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

GABA-A R beta 3 Antibody (S87-25) Summary

Immunogen
Fusion protein amino acids 370-433 of mouse GABA-A-R-Beta3.
Localization
Cell Membrane, Cell Junction, Synapse, Postsynaptic Cell Membrane
Specificity
Detects approx 55kDa. No cross-reactivity against GABA-A-R-Beta 2 or -Beta1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GABRB3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000
Application Notes
1 ug/ml of GABA-A Receptor Beta3 Antibody was sufficient for detection of Beta3 GABA receptor in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-47613.

Reactivity Notes

Based on homology, It's predicted to detect Human and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for GABA-A R beta 3 Antibody (S87-25)

  • ECA5
  • GABA A R beta 3
  • GABA(A) receptor beta-3 subunit
  • GABA(A) receptor subunit beta-3
  • GABAA receptor beta-3 subunit
  • GABA-alpha receptor beta-2 subunit
  • GABAAR beta 3
  • GABAARb3
  • GABRB3
  • gamma-aminobutyric acid (GABA) A receptor, beta 3
  • gamma-aminobutyric acid receptor subunit beta-3
  • MGC9051

Background

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GABA-A R beta 3 Antibody (NBP1-47613)(1)

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GABRB3
Entrez
Uniprot